ArticleComparative medicine2010
Assessment of rpoB and 16S rRNA genes as targets for PCR-based identification of Pasteurella pneumotropica.
Article in Comparative medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- Why Is the Current Monitoring of Rodentibacter spp. Exclusively by Molecular Methods Insufficient?Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- Experimental colonization with H. hepaticus, S. aureus and R. pneumotropicus does not influence the metabolic response to high-fat diet or incretin-analogues in wildtype SOPF mice.Molecular metabolism · 2024Article
- The Likelihood of Misidentifying Rodent Pasteurellaceae by Using Results from a Single PCR Assay.Journal of the American Association for Laboratory Animal Science : JAALAS · 2019Article
- Sensitive and immunogen-specific serological detection of Rodentibacter pneumotropicus infections in mice.BMC microbiology · 2019Article
- Article
- Exhaust Air Dust Monitoring is Superior to Soiled Bedding Sentinels for the Detection of Pasteurella pneumotropica in Individually Ventilated Cage Systems.Journal of the American Association for Laboratory Animal Science : JAALAS · 2016Article
- High-Resolution Melting Curve Analysis for Identification of Pasteurellaceae Species in Experimental Animal Facilities.PloS one · 2015Article
- Characterization of Biofilm Formation in [Pasteurella] pneumotropica and [Actinobacillus] muris Isolates of Mouse Origin.PloS one · 2015Article
- Elimination of Pasteurella pneumotropica from a mouse barrier facility by using a modified enrofloxacin treatment regimen.Journal of the American Association for Laboratory Animal Science : JAALAS · 2014Article
- Efficacy of direct detection of pathogens in naturally infected mice by using a high-density PCR array.Journal of the American Association for Laboratory Animal Science : JAALAS · 2013Article
- Pathogenicity and genetic variation of 3 strains of Corynebacterium bovis in immunodeficient mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2013Article
- Comparative metagenomics of toxic freshwater cyanobacteria bloom communities on two continents.PloS one · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diagnosis of Pasteurella pneumotropica in laboratory animals relies on isolation of the organism, biochemical characterization, and, more recently, DNA-based diagnostic methods. 16S rRNA and rpoB gene sequences were examined for development of a real-time PCR assay. Partial sequencing of rpoB (456 bp) and 16S rRNA (1368 bp) of Pasteurella pneumotropica isolates identified by microbiologic and biochemical assays indicated that either gene sequence can be used to distinguish P. pneumotropica from other members of the Pasteurellaceae family. However, alignment of rpoB sequences from the Pasteurella pneumotropica Heyl (15 sequences) and Jawetz (16 sequences) biotypes with other Pasteurellaceae sequences from GenBank indicated that although rpoB DNA sequencing could be used for diagnosis, development of diagnostic primers and probes would be difficult, because the sequence variability between Heyl and Jawetz biotypes is not clustered in any particular region of the rpoB sequence. In contrast, alignment of 16S rRNA sequences revealed a region with unique and stable nucleotide motifs sufficient to permit development of a specific fluorogenic real-time PCR assay to confirm P. pneumotropica isolated by culture and to differentiate Heyl and Jawetz biotypes.
Indexed as
Identifiers
21262128PMC3002101W1867997285What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.