ArticleMicrobial biotechnology2008
Induction of natural competence in Bacillus cereus ATCC14579.
Article in Microbial biotechnology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- Simultaneous Expression of Recombinant Cellulase and Protease in An IndigenousIranian journal of biotechnology · 2024Article
- BsuMI regulates DNA transformation in Bacillus subtilis besides the defense system and the constructed strain with BsuMI-absence is applicable as a universal transformation platform for wild-type Bacillus.Microbial cell factories · 2024Article
- Precision Genome Engineering inACS synthetic biology · 2023Article
- Genomic versus Plasmid-Borne Expression of Germinant Receptor Proteins in Bacillus cereus Strain 14579Microorganisms · 2022Article
- A facile and robust T7-promoter-based high-expression of heterologous proteins in Bacillus subtilis.Bioresources and bioprocessing · 2022Article
- Fluorescence in situ hybridization (FISH) and cell sorting of living bacteria.Scientific reports · 2019Article
- Natural DNA Transformation Is Functional in Lactococcus lactis subsp. cremoris KW2.Applied and environmental microbiology · 2017Article
- Silently transformable: the many ways bacteria conceal their built-in capacity of genetic exchange.Current genetics · 2017Review
- Sustainable biorefining in wastewater by engineered extreme alkaliphile Bacillus marmarensis.Scientific reports · 2016Article
- The comER Gene Plays an Important Role in Biofilm Formation and Sporulation in both Bacillus subtilis and Bacillus cereus.Frontiers in microbiology · 2016Article
- The Role of DNA Restriction-Modification Systems in the Biology of Bacillus anthracis.Frontiers in microbiology · 2016Review
- Effects of Genic Base Composition on Growth Rate in G+C-rich Genomes.G3 (Bethesda, Md.) · 2015Article
- Natural transformation of Thermotoga sp. strain RQ7.BMC biotechnology · 2014Article
- Bacterial transformation: distribution, shared mechanisms and divergent control.Nature reviews. Microbiology · 2014Review
- Establishment of a markerless mutation delivery system in Bacillus subtilis stimulated by a double-strand break in the chromosome.PloS one · 2013Article
- Article
- Expression of a cryptic secondary sigma factor gene unveils natural competence for DNA transformation in Staphylococcus aureus.PLoS pathogens · 2012Article
- Bacillus anthracis sin locus and regulation of secreted proteases.Journal of bacteriology · 2011Article
- Genetic variation and linkage disequilibrium in Bacillus anthracis.Scientific reports · 2011Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Natural competence is the ability of certain microbes to take up exogenous DNA from the environment and integrate it in their genome. Competence development has been described for a variety of bacteria, but has so far not been shown to occur in Bacillus cereus. However, orthologues of most proteins involved in natural DNA uptake in Bacillus subtilis could be identified in B. cereus. Here, we report that B. cereus ATCC14579 can become naturally competent. When expressing the B. subtilis ComK protein using an IPTG-inducible system in B. cereus ATCC14579, cells grown in minimal medium displayed natural competence, as either genomic DNA or plasmid DNA was shown to be taken up by the cells and integrated into the genome or stably maintained respectively. This work proves that a sufficient structural system for DNA uptake exists in B. cereus. Bacillus cereus can be employed as a model system to investigate the mechanism of DNA uptake in related bacteria such as Bacillus anthracis and Bacillus thuringiensis. Moreover, natural competence provides an important tool for biotechnology, as it will allow more efficient transformation of B. cereus and related organisms, e.g. to knockout genes in a high-throughput way.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.