ArticleMicrobial biotechnology2008
Development of a LytE-based high-density surface display system in Bacillus subtilis.
Article in Microbial biotechnology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Repurposing the bacterial surface display technology for drug delivery.Advanced drug delivery reviews · 2025Review
- Bacillus subtilis surface display technology: applications in bioprocessing and sustainable manufacturing.Biotechnology for biofuels and bioproducts · 2025Review
- Evidence that glycopolymer transferases promote peptidoglycan hydrolysis inbioRxiv : the preprint server for biology · 2025Article
- Quantitative measurement of cell-surface displayed proteins based on split-GFP assembly.Microbial cell factories · 2024Article
- Dynamics of cell wall-binding proteins at a single molecule level:Molecular biology of the cell · 2024Article
- Mucosal and systemic immune responses induced by intranasal immunization of recombinant Bacillus subtilis expressing the P97R1, P46 antigens of Mycoplasma hyopneumoniae.Bioscience reports · 2019Article
- A bio-coupling approach using a dextran-binding domain to immobilize an engineered streptavidin to Sephadex for easy preparation of affinity matrix.Scientific reports · 2019Article
- Stabilizing displayed proteins on vegetative Bacillus subtilis cells.Applied microbiology and biotechnology · 2018Article
- A simple approach for preparation of affinity matrices: Simultaneous purification and reversible immobilization of a streptavidin mutein to agarose matrix.Scientific reports · 2017Article
- Exploiting the peptidoglycan-binding motif, LysM, for medical and industrial applications.Applied microbiology and biotechnology · 2014Review
- The structure of the SBP-Tag-streptavidin complex reveals a novel helical scaffold bridging binding pockets on separate subunits.Acta crystallographica. Section D, Biological crystallography · 2013Article
- Enhanced microbial utilization of recalcitrant cellulose by an ex vivo cellulosome-microbe complex.Applied and environmental microbiology · 2012Article
- Functional characterization and localization of a Bacillus subtilis sortase and its substrate and use of this sortase system to covalently anchor a heterologous protein to the B. subtilis cell wall for surface display.Journal of bacteriology · 2012Article
- Energy, heat, flavours and aromas of Microbial Biotechnology.Microbial biotechnology · 2008Article
- Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The three N-terminal, tandemly arranged LysM motifs from a Bacillus subtilis cell wall hydrolase, LytE, formed a cell wall-binding module. This module, designated CWBM(LytE), was demonstrated to have tight cell wall-binding capability and could recognize two classes of cell wall binding sites with fivefold difference in affinity. The lower-affinity sites were approximately three times more abundant. Fusion proteins with β-lactamase attached to either the N- or C-terminal end of CWBM(LytE) showed lower cell wall-binding affinity. The number of the wall-bound fusion proteins was less than that of CWBM(LytE). These effects were less dramatic with CWBM(LytE) at the N-terminal end of the fusion. Both CWBM(LytE) and β-lactamase were essentially functional whether they were at the N- or C-terminal end of the fusion. In the optimal case, 1.2 × 10(7) molecules could be displayed per cell. As cells overproducing CWBM(LytE) and its fusions formed filamentous cells (with an average of nine individual cells per filamentous cell), 1.1 × 10(8)β-lactamase molecules could be displayed per filamentous cell. Overproduced CWBM(LytE) and its fusions were distributed on the entire cell surface. Surface exposure and accessibility of these proteins were confirmed by immunofluorescence microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.