ArticleMicrobial biotechnology2008
Massively parallel pathogen identification using high-density microarrays.
Article in Microbial biotechnology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Revisiting the genetic diversity of emerging hantaviruses circulating in Europe using a pan-viral resequencing microarray.Scientific reports · 2019Article
- Development and Assessment of a Diagnostic DNA Oligonucleotide Microarray for Detection and Typing of Meningitis-Associated Bacterial Species.High-throughput · 2018Article
- Accuracy of Different Bioinformatics Methods in Detecting Antibiotic Resistance and Virulence Factors from Staphylococcus aureus Whole-Genome Sequences.Journal of clinical microbiology · 2018Article
- A resequencing pathogen microarray method for high-throughput molecular diagnosis of multiple etiologies associated with central nervous system infection.Archives of virology · 2017Article
- Characterization of sal(A), a novel gene responsible for lincosamide and streptogramin A resistance in Staphylococcus sciuri.Antimicrobial agents and chemotherapy · 2014Article
- Resequencing microarray technology for genotyping human papillomavirus in cervical smears.PloS one · 2014Article
- The human virome: new tools and concepts.Trends in microbiology · 2013Review
- Article
- Differentiation of the seven major lyssavirus species by oligonucleotide microarray.Journal of clinical microbiology · 2012Article
- Recurrent Breast Abscesses due to Corynebacterium kroppenstedtii, a Human Pathogen Uncommon in Caucasian Women.Case reports in infectious diseases · 2012Article
- Microbial Diagnostic Microarrays for the Detection and Typing of Food- and Water-Borne (Bacterial) Pathogens.Microarrays (Basel, Switzerland) · 2011Review
- Application of a broad-range resequencing array for detection of pathogens in desert dust samples from Kuwait and Iraq.Applied and environmental microbiology · 2011Article
- Reconstructed ancestral sequences improve pathogen identification using resequencing DNA microarrays.PloS one · 2010Article
- More accurate insight into the incidence of human rabies in developing countries through validated laboratory techniques.PLoS neglected tropical diseases · 2010Review
- Use of consensus sequences for the design of high density resequencing microarrays: the influenza virus paradigm.BMC genomics · 2010Article
- Application of broad-spectrum resequencing microarray for genotyping rhabdoviruses.Journal of virology · 2010Article
- Article
- Testing and validation of high density resequencing microarray for broad range biothreat agents detection.PloS one · 2009Article
- Development and application of the active surveillance of pathogens microarray to monitor bacterial gene flux.BMC microbiology · 2008Article
- Phi29 polymerase based random amplification of viral RNA as an alternative to random RT-PCR.BMC molecular biology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
20 authors at 4 institutions in 2 countries.
Funding
Abstract
Identification of microbial pathogens in clinical specimens is still performed by phenotypic methods that are often slow and cumbersome, despite the availability of more comprehensive genotyping technologies. We present an approach based on whole-genome amplification and resequencing microarrays for unbiased pathogen detection. This 10 h process identifies a broad spectrum of bacterial and viral species and predicts antibiotic resistance and pathogenicity and virulence profiles. We successfully identify a variety of bacteria and viruses, both in isolation and in complex mixtures, and the high specificity of the microarray distinguishes between different pathogens that cause diseases with overlapping symptoms. The resequencing approach also allows identification of organisms whose sequences are not tiled on the array, greatly expanding the repertoire of identifiable organisms and their variants. We identify organisms by hybridization of their DNA in as little as 1-4 h. Using this method, we identified Monkeypox virus and drug-resistant Staphylococcus aureus in a skin lesion taken from a child suspected of an orthopoxvirus infection, despite poor transport conditions of the sample, and a vast excess of human DNA. Our results suggest this technology could be applied in a clinical setting to test for numerous pathogens in a rapid, sensitive and unbiased manner.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.