ArticleBiochemistry2011
Large favorable enthalpy changes drive specific RNA recognition by RNA recognition motif proteins.
Article in Biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Structure specific recognition of telomeric repeats containing RNA by the RGG-box of hnRNPA1.Nucleic acids research · 2020Article
- Exploring the suitability of RanBP2-type Zinc Fingers for RNA-binding protein design.Scientific reports · 2019Article
- RGG-box in hnRNPA1 specifically recognizes the telomere G-quadruplex DNA and enhances the G-quadruplex unfolding ability of UP1 domain.Nucleic acids research · 2018Article
- Comparative analyses of the thermodynamic RNA binding signatures of different types of RNA recognition motifs.Nucleic acids research · 2017Article
- Article
- SF1 Phosphorylation Enhances Specific Binding to U2AFBiophysical journal · 2016Article
- Unmasking the U2AF homology motif family: a bona fide protein-protein interaction motif in disguise.RNA (New York, N.Y.) · 2016Review
- A Structure-free Method for Quantifying Conformational Flexibility in proteins.Scientific reports · 2016Article
- Rbfox proteins regulate microRNA biogenesis by sequence-specific binding to their precursors and target downstream Dicer.Nucleic acids research · 2016Article
- Article
- Thermodynamic and phylogenetic insights into hnRNP A1 recognition of the HIV-1 exon splicing silencer 3 element.Biochemistry · 2014Article
- The binding of TIA-1 to RNA C-rich sequences is driven by its C-terminal RRM domain.RNA biology · 2014Article
- Structural basis of mRNA recognition and cleavage by toxin MazF and its regulation by antitoxin MazE in Bacillus subtilis.Molecular cell · 2013Article
- High-affinity interaction of hnRNP A1 with conserved RNA structural elements is required for translation and replication of enterovirus 71.RNA biology · 2013Article
- Thermodynamic studies of a series of homologous HIV-1 TAR RNA ligands reveal that loose binders are stronger Tat competitors than tight ones.Nucleic acids research · 2013Article
- The structure and selectivity of the SR protein SRSF2 RRM domain with RNA.Nucleic acids research · 2012Article
- Three RNA recognition motifs participate in RNA recognition and structural organization by the pro-apoptotic factor TIA-1.Journal of molecular biology · 2012Article
- Kinked β-strands mediate high-affinity recognition of mRNA targets by the germ-cell regulator DAZL.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
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Authors and funding
3 authors.
Funding
Abstract
The RNA recognition motif (RRM) is a prevalent class of RNA binding domains. Although a number of RRM/RNA structures have been determined, thermodynamic analyses are relatively uncommon. Here, we use isothermal titration calorimetry to characterize single-stranded (ss)RNA binding by four representative RRM-containing proteins: (i) U2AF(65), (ii) SXL, (iii) TIA-1, and (iv) PAB. In all cases, ssRNA binding is accompanied by remarkably large favorable enthalpy changes (-30 to -60 kcal mol(-1)) and unfavorable entropy changes. Alterations of key RRM residues and binding sites indicate that under the nearly physiological conditions of these studies, large thermodynamic changes represent a signature of specific ssRNA recognition by RRMs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.