ArticleBiomedical optics express2010
High-throughput FCS using an LCOS spatial light modulator and an 8 × 1 SPAD array.
Article in Biomedical optics express, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 79 citations in OpenAlex.
- Segmented fluorescence correlation spectroscopy (FCS) on a commercial laser scanning microscope.Scientific reports · 2024Article
- Performance of Active-Quenching SPAD Array Based on the Tri-State Gates of FPGA and Packaged with Bare Chip Stacking.Sensors (Basel, Switzerland) · 2023Article
- Computational Proposal for Tracking Multiple Molecules in a Multifocus Confocal Setup.ACS photonics · 2022Article
- Rapid ensemble measurement of protein diffusion and probe blinking dynamics in cells.Biophysical reports · 2021Article
- Confocal-based fluorescence fluctuation spectroscopy with a SPAD array detector.Light, science & applications · 2021Article
- Custom silicon technology for SPAD-arrays with red-enhanced sensitivity and low timing jitter.Optics express · 2021Article
- High-throughput fluorescence correlation spectroscopy enables analysis of surface components of cell-derived vesicles.Analytical and bioanalytical chemistry · 2020Article
- High-throughput, multi-parametric, and correlative fluorescence lifetime imaging.Methods and applications in fluorescence · 2020Review
- High-throughput smFRET analysis of freely diffusing nucleic acid molecules and associated proteins.Methods (San Diego, Calif.) · 2019Article
- Optical crosstalk in SPAD arrays for high-throughput single-molecule fluorescence spectroscopy.Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment · 2018Article
- Multifocal multiphoton volumetric imaging approach for high-speed time-resolved Förster resonance energy transfer imaging in vivo.Optics letters · 2018Article
- Widefield High Frame Rate Single-Photon SPAD Imagers for SPIM-FCS.Biophysical journal · 2018Article
- 48-spot single-molecule FRET setup with periodic acceptor excitation.The Journal of chemical physics · 2018Article
- Local raster image correlation spectroscopy generates high-resolution intracellular diffusion maps.Communications biology · 2018Article
- Multispot single-molecule FRET: High-throughput analysis of freely diffusing molecules.PloS one · 2017Article
- High-voltage integrated active quenching circuit for single photon count rate up to 80 Mcounts/s.Optics express · 2016Article
- Silicon technologies for arrays of Single Photon Avalanche Diodes.Proceedings of SPIE--the International Society for Optical Engineering · 2016Article
- Sub-10 nm near-field localization by plasmonic metal nanoaperture arrays with ultrashort light pulses.Scientific reports · 2015Article
- Multifocal fluorescence microscope for fast optical recordings of neuronal action potentials.Biophysical journal · 2015Article
- A high speed multifocal multiphoton fluorescence lifetime imaging microscope for live-cell FRET imaging.Biomedical optics express · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
We present a novel approach to high-throughput Fluorescence Correlation Spectroscopy (FCS) which enables us to obtain one order of magnitude improvement in acquisition time. Our approach utilizes a liquid crystal on silicon spatial light modulator to generate dynamically adjustable focal spots, and uses an eight-pixel monolithic single-photon avalanche photodiode array. We demonstrate the capabilities of this system by showing FCS of Rhodamine 6G under various viscosities, and by showing that, with proper calibration of each detection channel, one order of magnitude improvement in acquisition speed is obtained. More generally, our approach will allow higher throughput single-molecule studies to be performed.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.