ArticleApplied and environmental microbiology2011
Prevalence and characterization of non-O157 shiga toxin-producing Escherichia coli isolates from commercial ground beef in the United States.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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63 citing papers in PubMed, 219 citations in OpenAlex.
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- O55 Polysaccharides Are Good Antigen Targets for the Formulation of Vaccines against O55 STEC and Capsulated aEPEC Strains.Pathogens (Basel, Switzerland) · 2022Article
- Presence and Virulence Characteristics of Shiga ToxinFoodborne pathogens and disease · 2022Article
- Pathogenomes and variations in Shiga toxin production among geographically distinct clones ofMicrobial genomics · 2022Article
- Whole-genome characterization of hemolytic uremic syndrome-causing Shiga toxin-producingVirulence · 2021Article
- Diversity of Non-O157 Shiga Toxin-ProducingAnimals : an open access journal from MDPI · 2021Article
- Locus of Heat Resistance (LHR) in Meat-Borne Escherichia coli: Screening and Genetic Characterization.Applied and environmental microbiology · 2021Article
- First Isolation and Molecular Characterization ofFrontiers in veterinary science · 2021Article
- Improved Genomic Identification, Clustering, and Serotyping of Shiga Toxin-ProducingFrontiers in cellular and infection microbiology · 2021Article
- Seasonal Prevalence of Shiga Toxin-Producing Escherichia coli on Pork Carcasses for Three Steps of the Harvest Process at Two Commercial Processing Plants in the United States.Applied and environmental microbiology · 2020Article
- Genome Sequences of Uncommon Shiga Toxin-Producing Escherichia coli Serotypes.Microbiology resource announcements · 2020Article
- Quantitative risk assessment of haemolytic uremic syndrome associated with beef consumption in Argentina.PloS one · 2020Article
- Comparative Transcriptomics of Shiga Toxin-Producing and CommensalFrontiers in cellular and infection microbiology · 2020Article
- Molecular profiling and antimicrobial resistance of Shiga toxin-producing Escherichia coli O26, O45, O103, O121, O145 and O157 isolates from cattle on cow-calf operations in South Africa.Scientific reports · 2019Article
- Occurrence and antimicrobial resistance of E. coli non-O157 isolated from beef in Mato Grosso, Brazil.Tropical animal health and production · 2019Article
- Presence of Shiga Toxin-ProducingMicroorganisms · 2018Article
- Variability in CharacterizingMicroorganisms · 2018Article
- Review
3 more citing papers are in PubMed but not listed here.
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2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Escherichia coli O157:H7 is a Shiga toxin (stx)-producing E. coli (STEC) strain that has been classified as an adulterant in U.S. beef. However, numerous other non-O157 STEC strains are associated with diseases of various severities and have become an increasing concern to the beef industry, regulatory officials, and the public. This study reports on the prevalence and characterization of non-O157 STEC in commercial ground beef samples (n = 4,133) obtained from numerous manufacturers across the United States over a period of 24 months. All samples were screened by DNA amplification for the presence of Shiga toxin genes, which were present in 1,006 (24.3%) of the samples. Then, culture isolation of an STEC isolate from all samples that contained stx(1) and/or stx(2) was attempted. Of the 1,006 positive ground beef samples screened for stx, 300 (7.3% of the total of 4,133) were confirmed to have at least one strain of STEC present by culture isolation. In total, 338 unique STEC isolates were recovered from the 300 samples that yielded an STEC isolate. All unique STEC isolates were serotyped and were characterized for the presence of known virulence factors. These included Shiga toxin subtypes, intimin subtypes, and accessory virulence factors related to adherence (saa, iha, lifA), toxicity (cnf, subA, astA), iron acquisition (chuA), and the presence of the large 60-MDa virulence plasmid (espP, etpD, toxB, katP, toxB). The isolates were also characterized by use of a pathogenicity molecular risk assessment (MRA; based on the presence of various O-island nle genes). Results of this characterization identified 10 STEC isolates (0.24% of the 4,133 total) that may be considered a significant food safety threat, defined by the presence of eae, subA, and nle genes.
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