ArticleJournal of bacteriology2011
The MreB-like protein Mbl of Streptomyces coelicolor A3(2) depends on MreB for proper localization and contributes to spore wall synthesis.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- A histidine pseudokinase modulates polar growth and cell shape in Streptomyces venezuelae.Communications biology · 2026Article
- Streptomyces uses both polar and dispersed cell wall synthesis during exploratory growth.Nature microbiology · 2025Article
- Age-Dependent Pleomorphism inMicroorganisms · 2025Article
- Pangenomes of human gut microbiota uncover links between genetic diversity and stress response.Cell host & microbe · 2024Article
- Intracellular Phage Tail-Like Nanostructures Affect Susceptibility of Streptomyces lividans to Osmotic Stress.mSphere · 2023Article
- The evolution of morphological development is congruent with the species phylogeny in the genusFrontiers in microbiology · 2023Article
- Isolation, Characterization, and Genome Sequence Analysis of a Novel Lytic Phage, Xoo-sp15 Infecting Xanthomonas oryzae pv. oryzae.Current microbiology · 2021Article
- A Comprehensive Evolutionary Scenario of Cell Division and Associated Processes in the Firmicutes.Molecular biology and evolution · 2021Article
- Structural, Metabolic and Evolutionary Comparison of Bacterial Endospore and Exospore Formation.Frontiers in microbiology · 2021Review
- A Novel Two-Component System, Encoded by the sFrontiers in microbiology · 2019Article
- A trehalose biosynthetic enzyme doubles as an osmotic stress sensor to regulate bacterial morphogenesis.PLoS genetics · 2017Article
- A Waking Review: Old and Novel Insights into the Spore Germination inFrontiers in microbiology · 2017Review
- Polydiglycosylphosphate Transferase PdtA (SCO2578) of Streptomyces coelicolor A3(2) Is Crucial for Proper Sporulation and Apical Tip Extension under Stress Conditions.Applied and environmental microbiology · 2016Article
- Taxonomy, Physiology, and Natural Products of Actinobacteria.Microbiology and molecular biology reviews : MMBR · 2016Review
- Control of Morphological Differentiation of Streptomyces coelicolor A3(2) by Phosphorylation of MreC and PBP2.PloS one · 2015Article
- Interplay between two bacterial actin homologs, MamK and MamK-Like, is required for the alignment of magnetosome organelles in Magnetospirillum magneticum AMB-1.Journal of bacteriology · 2014Article
- Developmental biology of Streptomyces from the perspective of 100 actinobacterial genome sequences.FEMS microbiology reviews · 2014Review
- A sporulation-specific, sigF-dependent protein, SspA, affects septum positioning in Streptomyces coelicolor.Molecular microbiology · 2014Article
- Modes of cell wall growth differentiation in rod-shaped bacteria.Current opinion in microbiology · 2013Review
- Structure-function relationships of two paralogous single-stranded DNA-binding proteins from Streptomyces coelicolor: implication of SsbB in chromosome segregation during sporulation.Nucleic acids research · 2013Article
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8 authors.
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Abstract
Most bacteria with a rod-shaped morphology contain an actin-like cytoskeleton consisting of MreB polymers, which form helical spirals underneath the cytoplasmic membrane to direct peptidoglycan synthesis for the elongation of the cell wall. In contrast, MreB of Streptomyces coelicolor is not required for vegetative growth but has a role in sporulation. Besides MreB, S. coelicolor encodes two further MreB-like proteins, Mbl and SCO6166, whose function is unknown. Whereas MreB and Mbl are highly similar, SCO6166 is shorter, lacking the subdomains IB and IIB of actin-like proteins. Here, we showed that MreB and Mbl are not functionally redundant but cooperate in spore wall synthesis. Expression analysis by semiquantitative reverse transcription-PCR revealed distinct expression patterns. mreB and mbl are induced predominantly during morphological differentiation. In contrast, sco6166 is strongly expressed during vegetative growth but switched off during sporulation. All genes could be deleted without affecting viability. Even a ΔmreB Δmbl double mutant was viable. Δsco6166 had a wild-type phenotype. ΔmreB, Δmbl, and ΔmreB Δmbl produced swollen, prematurely germinating spores that were sensitive to various kinds of stress, suggesting a defect in spore wall integrity. During aerial mycelium formation, an Mbl-mCherry fusion protein colocalized with an MreB-enhanced green fluorescent protein (MreB-eGFP) fusion protein at the sporulation septa. Whereas MreB-eGFP localized properly in the Δmbl mutant, Mbl-mCherry localization depended on the presence of a functional MreB protein. Our results revealed that MreB and Mbl cooperate in the synthesis of the thickened spore wall, while SCO6166 has a nonessential function during vegetative growth.
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