ArticleJournal of bacteriology2011
Corynebacterium glutamicum as a host for synthesis and export of D-Amino Acids.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 55 citations in OpenAlex.
- Enhancement and balance of 1,4-butanediamine biosynthesis in Escherichia coli based on modular metabolic engineering.Journal, genetic engineering & biotechnology · 2026Article
- D-serine supplementation is associated with mucosal-prioritized rumen development and propionate-enriched fermentation with selective microbial shifts in pre-weaning Hu lambs.Frontiers in microbiology · 2026Article
- Impact of exporter proteins and their engineering on the productivity of Corynebacterium.Applied microbiology and biotechnology · 2025Review
- Metabolic Engineering of Corynebacterium glutamicum for High-Level Production of 1,5-Pentanediol, a C5 Diol Platform Chemical.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- l-Alanine Exporter AlaE Functions as One of the d-Alanine Exporters inInternational journal of molecular sciences · 2023Article
- Customized chitooligosaccharide production-controlling their lengthFrontiers in bioengineering and biotechnology · 2022Article
- Exploitation of Ultrasound Technique for Enhancement of Microbial Metabolites Production.Molecules (Basel, Switzerland) · 2020Review
- Recent Advances of L-ornithine Biosynthesis in Metabolically EngineeredFrontiers in bioengineering and biotechnology · 2019Review
- Metabolite secretion in microorganisms: the theory of metabolic overflow put to the test.Metabolomics : Official journal of the Metabolomic Society · 2018Review
- Cofactor recycling for co-production of 1,3-propanediol and glutamate by metabolically engineered Corynebacterium glutamicum.Scientific reports · 2017Article
- Ciprofloxacin triggered glutamate production by Corynebacterium glutamicum.BMC microbiology · 2016Article
- Characterization of a Unique Pathway for 4-Cresol Catabolism Initiated by Phosphorylation in Corynebacterium glutamicum.The Journal of biological chemistry · 2016Article
- Ribosome binding site libraries and pathway modules for shikimic acid synthesis with Corynebacterium glutamicum.Microbial cell factories · 2015Article
- A chromosomally encoded T7 RNA polymerase-dependent gene expression system for Corynebacterium glutamicum: construction and comparative evaluation at the single-cell level.Microbial biotechnology · 2015Article
- Characterization of 3-phosphoglycerate kinase from Corynebacterium glutamicum and its impact on amino acid production.BMC microbiology · 2014Article
- Visualization of imbalances in sulfur assimilation and synthesis of sulfur-containing amino acids at the single-cell level.Applied and environmental microbiology · 2013Article
- Complex regulation of the phosphoenolpyruvate carboxykinase gene pck and characterization of its GntR-type regulator IolR as a repressor of myo-inositol utilization genes in Corynebacterium glutamicum.Journal of bacteriology · 2013Article
- Glycerol as a substrate for aerobic succinate production in minimal medium with Corynebacterium glutamicum.Microbial biotechnology · 2013Article
- Reductive whole-cell biotransformation with Corynebacterium glutamicum: improvement of NADPH generation from glucose by a cyclized pentose phosphate pathway using pfkA and gapA deletion mutants.Applied microbiology and biotechnology · 2013Article
- Two-component signal transduction in Corynebacterium glutamicum and other corynebacteria: on the way towards stimuli and targets.Applied microbiology and biotechnology · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A number of d-amino acids occur in nature, and there is growing interest in their function and metabolism, as well as in their production and use. Here we use the well-established l-amino-acid-producing bacterium Corynebacterium glutamicum to study whether d-amino acid synthesis is possible and whether mechanisms for the export of these amino acids exist. In contrast to Escherichia coli, C. glutamicum tolerates d-amino acids added extracellularly. Expression of argR (encoding the broad-substrate-specific racemase of Pseudomonas taetrolens) with its signal sequence deleted results in cytosolic localization of ArgR in C. glutamicum. The isolated enzyme has the highest activity with lysine (100%) but also exhibits activity with serine (2%). Upon overexpression of argR in an l-arginine, l-ornithine, or l-lysine producer, equimolar mixtures of the d- and l-enantiomers accumulated extracellularly. Unexpectedly, argR overexpression in an l-serine producer resulted in extracellular accumulation of a surplus of d-serine (81 mM d-serine and 37 mM l-serine) at intracellular concentrations of 125 mM d-serine plus 125 mM l-serine. This points to a nonlimiting ArgR activity for intracellular serine racemization and to the existence of a specific export carrier for d-serine. Export of d-lysine relies fully on the presence of lysE, encoding the exporter for l-lysine, which is apparently promiscuous with respect to the chirality of lysine. These data show that d-amino acids can also be produced with C. glutamicum and that in special cases, due to specific carriers, even a preferential extracellular accumulation of this enantiomer is possible.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.