ArticleAnalytical biochemistry2011
Measuring protein synthesis using metabolic ²H labeling, high-resolution mass spectrometry, and an algorithm.
Article in Analytical biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- Kinetic Lipidomics: QuantifyingbioRxiv : the preprint server for biology · 2026Article
- Measuring HSD17β13 protein turnover in mouse liver with DBioanalysis · 2025Article
- Numbers of Exchangeable Hydrogens from LC-MS Data of Heavy Water Metabolically Labeled Samples.Journal of the American Society for Mass Spectrometry · 2024Article
- Metabolomic and proteomic applications to exercise biomedicine.Translational exercise biomedicine · 2024Review
- A high-fat diet increases hepatic mitochondrial turnover through restricted acetylation in a NAFLD mouse model.American journal of physiology. Endocrinology and metabolism · 2023Article
- Determining and interpreting protein lifetimes in mammalian tissues.Trends in biochemical sciences · 2023Review
- Utilizing Nonequilibrium Isotope Enrichments to Dramatically Increase Turnover Measurement Ranges in Single Biopsy Samples from Humans.Journal of proteome research · 2022Article
- Protein turnover models for LC-MS data of heavy water metabolic labeling.Briefings in bioinformatics · 2022Article
- Research Progress in the Medical Application of Heavy Water, Especially in the Field of DInternational journal of medical sciences · 2022Review
- Using Heavy Mass Isotopomers for Protein Turnover in Heavy Water Metabolic Labeling.Journal of proteome research · 2021Article
- Principles of stable isotope research - with special reference to protein metabolism.Clinical nutrition open science · 2021Article
- Measuring acetyl-CoA and acetylated histone turnover in vivo: Effect of a high fat diet.Analytical biochemistry · 2021Article
- Partial Isotope Profiles Are Sufficient for Protein Turnover Analysis Using Closed-Form Equations of Mass Isotopomer Dynamics.Analytical chemistry · 2020Article
- High-Resolution Mass Spectrometry for In Vivo Proteome Dynamics using Heavy Water Metabolic Labeling.International journal of molecular sciences · 2020Article
- Early Pro-Inflammatory Remodeling of HDL Proteome in a Model of Diet-Induced Obesity:International journal of molecular sciences · 2020Article
- Timepoint Selection Strategy for In Vivo Proteome Dynamics from Heavy Water Metabolic Labeling and LC-MS.Journal of proteome research · 2020Article
- Temporal Dynamics of High-Density Lipoprotein Proteome in Diet-Controlled Subjects with Type 2 Diabetes.Biomolecules · 2020Article
- Calculation of the Protein Turnover Rate Using the Number of IncorporatedAnalytical chemistry · 2019Article
- Proteome Dynamics from Heavy Water Metabolic Labeling and Peptide Tandem Mass Spectrometry.International journal of mass spectrometry · 2019Article
- Hepatic Mitochondrial Defects in a Nonalcoholic Fatty Liver Disease Mouse Model Are Associated with Increased Degradation of Oxidative Phosphorylation Subunits.Molecular & cellular proteomics : MCP · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
We recently developed a method for estimating protein dynamics in vivo with heavy water ((2)H(2)O) using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) [16], and we confirmed that (2)H labeling of many hepatic free amino acids rapidly equilibrated with body water. Although this is a reliable method, it required modest sample purification and necessitated the determination of tissue-specific amino acid labeling. Another approach for quantifying protein kinetics is to measure the (2)H enrichments of body water (precursor) and protein-bound amino acid or proteolytic peptide (product) and to estimate how many copies of deuterium are incorporated into a product. In the current study, we used nanospray linear trap Fourier transform ion cyclotron resonance mass spectrometry (LTQ FT-ICR MS) to simultaneously measure the isotopic enrichment of peptides and protein-bound amino acids. A mathematical algorithm was developed to aid the data processing. The most notable improvement centers on the fact that the precursor/product labeling ratio can be obtained by measuring the labeling of water and a protein (or peptide) of interest, thereby minimizing the need to measure the amino acid labeling. As a proof of principle, we demonstrate that this approach can detect the effect of nutritional status on albumin synthesis in rats given (2)H(2)O.
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