ArticleCurrent biology : CB2011
Redox-responsive zinc finger fidelity switch in homing endonuclease and intron promiscuity in oxidative stress.
Article in Current biology : CB, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- Group I introns: Structure, splicing and their applications in medical mycology.Genetics and molecular biology · 2024Article
- Distribution and Polymorphisms of Group I Introns in Mitochondrial Genes fromJournal of fungi (Basel, Switzerland) · 2023Article
- Stress-Induced Mutagenesis, Gambler Cells, and Stealth Targeting Antibiotic-Induced Evolution.mBio · 2022Review
- Comparative analysis of the complete mitochondrial genomes of four cordyceps fungi.Ecology and evolution · 2022Article
- The Coevolution of Fungal Mitochondrial Introns and Their Homing Endonucleases (GIY-YIG and LAGLIDADG).Genome biology and evolution · 2020Article
- What is mutation? A chapter in the series: How microbes "jeopardize" the modern synthesis.PLoS genetics · 2019Review
- Polymorphism in Mitochondrial Group I Introns amongFrontiers in microbiology · 2018Article
- Mobile self-splicing introns and inteins as environmental sensors.Current opinion in microbiology · 2017Review
- Stress-Induced Mutagenesis: Implications in Cancer and Drug Resistance.Annual review of cancer biology · 2017Article
- A Drosophila model identifies a critical role for zinc in mineralization for kidney stone disease.PloS one · 2015Article
- Bacterial group I introns: mobile RNA catalysts.Mobile DNA · 2014Article
- Structural insights into the N-terminal GIY-YIG endonuclease activity of Arabidopsis glutaredoxin AtGRXS16 in chloroplasts.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Regulatory control or oxidative damage? Proteomic approaches to interrogate the role of cysteine oxidation status in biological processes.Molecular & cellular proteomics : MCP · 2012Review
- Learning to live together: mutualism between self-splicing introns and their hosts.BMC biology · 2011Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
It is well understood how mobile introns home to allelic sites, but how they are stimulated to transpose to ectopic locations on an evolutionary timescale is unclear. Here we show that a group I intron can move to degenerate sites under oxidizing conditions. The phage T4 td intron endonuclease, I-TevI, is responsible for this infidelity. We demonstrate that I-TevI, which promotes mobility and is subject to autorepression and translational control, is also regulated posttranslationally by a redox mechanism. Redox regulation is exercised by a zinc finger (ZF) in a linker that connects the catalytic domain of I-TevI to the DNA binding domain. Four cysteines coordinate Zn(2+) in the ZF, which ensures that I-TevI cleaves its DNA substrate at a fixed distance, 23-25 nucleotides upstream of the intron insertion site. We show that the fidelity of I-TevI cleavage is controlled by redox-responsive Zn(2+) cycling. When the ZF is mutated, or after exposure of the wild-type I-TevI to H(2)O(2), intron homing to degenerate sites is increased, likely because of indiscriminate DNA cleavage. These results suggest a mechanism for rapid intron dispersal, joining recent descriptions of the activation of biomolecular processes by oxidative stress through cysteine chemistry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.