Evidence map›Paper›PMID 21255583›Full record

ArticleJournal of molecular biology2011

Single-molecule studies of transcription: from one RNA polymerase at a time to the gene expression profile of a cell.

Feng Wang, Eric C Greene

Abstract read
In one paragraph

Article in Journal of molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Dissecting the stochastic transcription initiation process in live Escherichia coli.DNA research : an international journal for rapid publication of reports on genes and genomes · 2016
    Article
  6. Article
  7. Article
  8. Review
  9. The bright future of single-molecule fluorescence imaging.Current opinion in chemical biology · 2014
    Review
  10. RNA polymerase: in search of promoters.Annals of the New York Academy of Sciences · 2013
    Review
  11. RNA polymerase approaches its promoter without long-range sliding along DNA.Proceedings of the National Academy of Sciences of the United States of America · 2013
    Article
  12. Article
  13. Dynamics of lesion processing by bacterial nucleotide excision repair proteins.Progress in molecular biology and translational science · 2012
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Feng WangDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
Eric C Greene

Funding

Elucidating the Mechanisms of DNA RecombinationR01GM074739 · NIGMS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI GREENE, ERIC C · 2006 to 2015
$2.7M
Visualizing the Dynamics of Chromatin and Chromatin Remodeling ProteinsR01GM082848 · NIGMS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI GREENE, ERIC C · 2008 to 2016
$2.4M
NIGMS NIH HHS GM074739NIGMS NIH HHS GM082848NIGMS NIH HHS R01 GM074739NIGMS NIH HHS R01 GM082848
6 · The paper itself

Abstract

Single-molecule techniques have emerged as powerful tools for deciphering mechanistic details of transcription and have yielded discoveries that would otherwise have been impossible to make through the use of more traditional biochemical and/or biophysical techniques. Here, we provide a brief overview of single-molecule techniques most commonly used for studying RNA polymerase and transcription. We then present specific examples of single-molecule studies that have contributed to our understanding of key mechanistic details for each different stage of the transcription cycle. Finally, we discuss emerging single-molecule approaches and future directions, including efforts to study transcription at the single-molecule level in living cells.

Indexed as

Gene Expression ProfilingTranscription, GeneticDNA-Directed RNA PolymerasesModels, BiologicalMolecular BiologyDNA-Directed RNA Polymerases

Identifiers

PMID21255583
PMCPMC3230130

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.