ArticleCell communication and signaling : CCS2011
Effector granules in human T lymphocytes: the luminal proteome of secretory lysosomes from human T cells.
Article in Cell communication and signaling : CCS, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Article
- Pretreatment with IL-15 and IL-18 rescues natural killer cells from granzyme B-mediated apoptosis after cryopreservation.Nature communications · 2024Article
- Separation of Single Core and Multicore Lytic Granules by Subcellular Fractionation and Immunoisolation.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Review
- ChronicAmerican journal of physiology. Heart and circulatory physiology · 2021Article
- Distinctive CD26 Expression on CD4 T-Cell Subsets.Biomolecules · 2021Article
- Intra- and Extracellular Effector Vesicles From Human T And NK Cells: Same-Same, but Different?Frontiers in immunology · 2021Review
- Galectins in Intra- and Extracellular Vesicles.Biomolecules · 2020Review
- Degranulation of human cytotoxic lymphocytes is a major source of proteolytically active soluble CD26/DPP4.Cellular and molecular life sciences : CMLS · 2020Article
- Molecular mechanism to recruit galectin-3 into multivesicular bodies for polarized exosomal secretion.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Quantitative Imaging Approaches to Study the CAR Immunological Synapse.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Review
- Preparing the lethal hit: interplay between exo- and endocytic pathways in cytotoxic T lymphocytes.Cellular and molecular life sciences : CMLS · 2017Review
- Cut to the chase: a review of CD26/dipeptidyl peptidase-4's (DPP4) entanglement in the immune system.Clinical and experimental immunology · 2016Review
- Protein networks and activation of lymphocytes.Current opinion in immunology · 2015Review
- An extended proteome map of the lysosomal membrane reveals novel potential transporters.Molecular & cellular proteomics : MCP · 2013Article
- Differential surface expression of ADAM10 and ADAM17 on human T lymphocytes and tumor cells.PloS one · 2013Article
- Article
- Meeting report: Signal transduction meets systems biology.Cell communication and signaling : CCS · 2012Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCytotoxic cells of the immune system have evolved a lysosomal compartment to store and mobilize effector molecules. In T lymphocytes and NK cells, the death factor FasL is one of the characteristic marker proteins of these so-called secretory lysosomes, which combine properties of conventional lysosomes and exocytotic vesicles. Although these vesicles are crucial for immune effector function, their protein content in T cells has so far not been investigated in detail.
resultsIn the present study, intact membranous vesicles were enriched from homogenates of polyclonally activated T cells and initially characterized by Western blotting and electron microscopic inspection. The vesicular fraction that contained the marker proteins of secretory lysosomes was subsequently analyzed by 2D electrophoresis and mass spectrometry. The proteome analysis and data evaluation revealed that 70% of the 397 annotated proteins had been associated with different lysosome-related organelles in previous proteome studies.
conclusionWe provide the first comprehensive proteome map of T cell-derived secretory lysosomes with only minor contaminations by cytosolic, nuclear or other proteins. This information will be useful to more precisely address the activation-dependent maturation and the specific distribution of effector organelles and proteins in individual T or NK cell populations in future studies.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.