ArticleMicrobial biotechnology2010
Controlling biofilm formation, prophage excision and cell death by rewiring global regulator H-NS of Escherichia coli.
Article in Microbial biotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 71 citations in OpenAlex.
- Global transmission and distribution of phage-encoded cholera toxin genes constrained by toxin-repression genes and anti-phage defense systems.The ISME journal · 2026Article
- KilR ofmSphere · 2025Article
- A transcription factor from the cryptic Escherichia coli Rac prophage controls both phage and host operons.Nucleic acids research · 2025Article
- Post-translational modifications of the nucleoid protein H-NS: sites, mechanisms, and regulatory cues.FEMS microbiology reviews · 2025Review
- Lethal perturbation of an Escherichia coli regulatory network is triggered by a restriction-modification system's regulator and can be mitigated by excision of the cryptic prophage Rac.Nucleic acids research · 2024Article
- Acetylation of xenogeneic silencer H-NS regulates biofilm development through the nitrogen homeostasis regulator in Shewanella.Nucleic acids research · 2024Article
- In vitro Activity of Cefepime/Avibactam Against Carbapenem ResistantInfection and drug resistance · 2023Article
- Degradation of gene silencer is essential for expression of foreign genes and bacterial colonization of the mammalian gut.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- RecT Affects Prophage Lifestyle and Host Core Cellular Processes in Pseudomonas aeruginosa.Applied and environmental microbiology · 2022Article
- Distinct heterochromatin-like domains promote transcriptional memory and silence parasitic genetic elements in bacteria.The EMBO journal · 2022Article
- Concerns with computational protein engineering programmes IPRO and OptMAVEn and metabolic pathway engineering programme optStoic.Open biology · 2021Article
- Proteomic and Transcriptomic Analyses Indicate Reduced Biofilm-Forming Abilities in Cefiderocol-ResistantFrontiers in microbiology · 2021Article
- Impact of Xenogeneic Silencing on Phage-Host Interactions.Journal of molecular biology · 2019Review
- Anti-bacterial activity of baicalin against APEC through inhibition of quorum sensing and inflammatory responses.Scientific reports · 2019Article
- Excisionase in Pf filamentous prophage controls lysis-lysogeny decision-making in Pseudomonas aeruginosa.Molecular microbiology · 2019Article
- Bacterial 'Grounded' Prophages: Hotspots for Genetic Renovation and Innovation.Frontiers in genetics · 2019Article
- Engineered global regulator H-NS improves the acid tolerance of E. coli.Microbial cell factories · 2018Article
- Article
- ImprovingBiotechnology for biofuels · 2017Article
- Cold adaptation regulated by cryptic prophage excision in Shewanella oneidensis.The ISME journal · 2016Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
The global regulator H-NS of Escherichia coli controls genes related to stress response, biofilm formation and virulence by recognizing curved DNA and by silencing acquired genes. Here, we rewired H-NS to control biofilm formation using protein engineering; H-NS variant K57N was obtained that reduces biofilm formation 10-fold compared with wild-type H-NS (wild-type H-NS increases biofilm formation whereas H-NS K57N reduces it). Whole-transcriptome analysis revealed that H-NS K57N represses biofilm formation through its interaction with the nucleoid-associated proteins Cnu and StpA and in the absence of these proteins, H-NS K57N was unable to reduce biofilm formation. Significantly, H-NS K57N enhanced the excision of defective prophage Rac while wild-type H-NS represses excision, and H-NS controlled only Rac excision among the nine resident E. coli K-12 prophages. Rac prophage excision not only led to the change in biofilm formation but also resulted in cell lysis through the expression of toxin HokD. Hence, the H-NS regulatory system may be evolved through a single-amino-acid change in its N-terminal oligomerization domain to control biofilm formation, prophage excision and apoptosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.