ArticleMicrobial biotechnology2010
Strategies for enhancing bioluminescent bacterial sensor performance by promoter region manipulation.
Article in Microbial biotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 66 citations in OpenAlex.
- Bioluminescent Microbial Bioreporters: A Personal Perspective.Biosensors · 2025Review
- The expression of different gene constructs in Escherichia coli SM lux biosensor after exposure to drugs.Scientific reports · 2024Article
- Bacterial Lux Biosensors in Genotoxicological Studies.Biosensors · 2023Article
- Organic persistent luminescence imaging for biomedical applications.Materials today. Bio · 2022Article
- Introduction of quorum sensing elements into bacterial bioreporter circuits enhances explosives' detection capabilities.Engineering in life sciences · 2022Article
- Direct and Indirect Chemiluminescence: Reactions, Mechanisms and Challenges.Molecules (Basel, Switzerland) · 2021Review
- The role of O-polysaccharide chain and complement resistance of Escherichia coli in mammary virulence.Veterinary research · 2020Article
- Ultrasound-Enhanced Chemiluminescence for Bioimaging.Frontiers in bioengineering and biotechnology · 2020Review
- A Smartphone-Based Whole-Cell Array Sensor for Detection of Antibiotics in Milk.Sensors (Basel, Switzerland) · 2019Article
- Dynamic Clustering of the Bacterial Sensory Kinase BaeS.PloS one · 2016Article
- Recent Advances in Genetic Technique of Microbial Report Cells and Their Applications in Cell Arrays.BioMed research international · 2015Review
- A bacterial reporter panel for the detection and classification of antibiotic substances.Microbial biotechnology · 2012Article
- Explorative probes and biomarkers, chronic Salmonella infections and future vaccines.Microbial biotechnology · 2012Article
- Are luminescent bacteria suitable for online detection and monitoring of toxic compounds in drinking water and its sources?Analytical and bioanalytical chemistry · 2011Review
- Bacterial genotoxicity bioreporters.Microbial biotechnology · 2010Review
- Microbial Biotechnology: biofuels, genotoxicity reporters and robust agro-ecosystems.Microbial biotechnology · 2010Article
- Strategies for enhancing bioluminescent bacterial sensor performance by promoter region manipulation.Bioengineered bugsArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bioluminescent bacterial sensors are based upon the fusion of bacterial bioluminescence (lux) genes, acting as a reporter element, to selected bacterial stress-response gene promoters. Depending upon the nature of the promoter, the resulting constructs react to diverse types of environmental stress, including the presence of toxic chemicals, by dose-dependant light emission. Two bacterial sensors, harbouring sulA::luxCDABE and grpE::luxCDABE fusions, activated by the model chemicals nalidixic acid (NA) and ethanol, respectively, were subjected to molecular manipulations of the promoter region, in order to enhance the intensity and speed of their response and lower their detection thresholds. By manipulating the length of the promoter-containing segment (both promoters), by introducing random or specific mutations in the promoter sequence or by duplicating the promoter sequence (sulA only), major improvements in sensor performance were obtained. Improvements included significantly enhanced sensitivity, earlier response times and an increase in signal intensity. The general approaches described herein may be of general applicability for optimizing bacterial sensor performance, regardless of the sensing or reporting elements employed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.