ArticleMicrobial biotechnology2010
Pleurotus ostreatus manganese-dependent peroxidase silencing impairs decolourization of Orange II.
Article in Microbial biotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 53 citations in OpenAlex.
- Article
- Seeking the Roles for Fungal Small-Secreted Proteins in Affecting Saprophytic Lifestyles.Frontiers in microbiology · 2020Review
- Manipulating the Expression of Small Secreted Protein 1 (Ssp1) Alters Patterns of Development and Metabolism in the White-Rot FungusApplied and environmental microbiology · 2019Article
- Enzymatic gene expression by Pleurotus tuoliensis (Bailinggu): differential regulation under low temperature induction conditions.World journal of microbiology & biotechnology · 2018Article
- A role for small secreted proteins (SSPs) in a saprophytic fungal lifestyle: Ligninolytic enzyme regulation in Pleurotus ostreatus.Scientific reports · 2017Article
- Limits of Versatility of Versatile Peroxidase.Applied and environmental microbiology · 2016Article
- Effects of calmodulin on expression of lignin-modifying enzymes in Pleurotus ostreatus.Current genetics · 2015Article
- How to enjoy laccases.Cellular and molecular life sciences : CMLS · 2015Review
- Sensitivity of Neurospora crassa to a marine-derived Aspergillus tubingensis anhydride exhibiting antifungal activity that is mediated by the MAS1 protein.Marine drugs · 2014Article
- Redundancy among manganese peroxidases in Pleurotus ostreatus.Applied and environmental microbiology · 2013Article
- Predominance of a versatile-peroxidase-encoding gene, mnp4, as demonstrated by gene replacement via a gene targeting system for Pleurotus ostreatus.Applied and environmental microbiology · 2012Article
- Release of Pleurotus ostreatus versatile-peroxidase from Mn2+ repression enhances anthropogenic and natural substrate degradation.PloS one · 2012Article
- Sugar (ribose), spice (peroxidase) and all things nice (laccase hair-dyes).Microbial biotechnology · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Decolourization of azo dyes by Pleurotus ostreatus, a white-rot fungus capable of lignin depolymerization and mineralization, is related to the ligninolytic activity of enzymes produced by this fungus. The capacity of P. ostreatus to decolourize the azo dye Orange II (OII) was dependent and positively co-linear to Mn(2+) concentration in the medium, and thus attributed to Mn(2+)-dependent peroxidase (MnP) activity. Based on the ongoing P. ostreatus genome deciphering project we identified at least nine genes encoding for MnP gene family members (mnp 1-9), of which only four (mnp 1-4) were previously known. Relative real-time PCR quantification analysis confirmed that all the nine genes are transcribed, and that Mn(2+) amendment results in a drastic increase in the transcript levels of the predominantly expressed MnP genes (mnp 3 and mnp 9), while decreasing versatile peroxidase gene transcription (mnp 4). A reverse genetics strategy based on silencing the P. ostreatus mnp 3 gene by RNAi was implemented. Knock-down of mnp 3 resulted in the reduction of fungal OII decolourization capacity, which was co-linear with marked silencing of the Mn(2+)-dependent peroxidase genes mnp 3 and mnp 9. This is the first direct genetic proof of an association between MnP gene expression levels and azo dye decolourization capacity in P. ostreatus, which may have significant implication on understanding the mechanisms governing lignin biodegradation. Moreover, this study has proven the applicability of RNAi as a tool for gene function studies in Pleurotus research.
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