ReviewMicrobial biotechnology2010
LOVely enzymes - towards engineering light-controllable biocatalysts.
Review in Microbial biotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 46 citations in OpenAlex.
- Activation of enzymatic catalysis via nucleic acid hybridization affords synergistic coupling of specificity, potency, and signal amplification.Nature communications · 2025Article
- Magnetizing Biotech-Advances in (In Vivo) Magnetic Enzyme Immobilization.Engineering in life sciences · 2025Review
- Machine Learning-Assisted Engineering of Light, Oxygen, Voltage Photoreceptor Adduct Lifetime.JACS Au · 2023Article
- Pharmaceuticals in the Aquatic Environment: A Review on Eco-Toxicology and the Remediation Potential of Algae.International journal of environmental research and public health · 2022Review
- Engineering a light-activated caspase-3 for precise ablation of neurons in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- A Native Threonine Coordinates Ordered Water to Tune Light-Oxygen-Voltage (LOV) Domain Photocycle Kinetics and Osmotic Stress Signaling in Trichoderma reesei ENVOY.The Journal of biological chemistry · 2016Article
- A critical element of the light-induced quaternary structural changes in YtvA-LOV.Protein science : a publication of the Protein Society · 2015Article
- A particular silent codon exchange in a recombinant gene greatly influences host cell metabolic activity.Microbial cell factories · 2015Article
- Exchange of single amino acids at different positions of a recombinant protein affects metabolic burden in Escherichia coli.Microbial cell factories · 2015Article
- Parallel use of shake flask and microtiter plate online measuring devices (RAMOS and BioLector) reduces the number of experiments in laboratory-scale stirred tank bioreactors.Journal of biological engineering · 2015Article
- Subcellular optogenetics - controlling signaling and single-cell behavior.Journal of cell science · 2015Review
- Tools for controlling protein interactions using light.Current protocols in cell biology · 2014Review
- Protein engineering: a new frontier for biological therapeutics.Current drug metabolism · 2014Review
- Review
- Engineering of bacterial phytochromes for near-infrared imaging, sensing, and light-control in mammals.Chemical Society reviews · 2013Review
- Optogenetic control of cell function using engineered photoreceptors.Biology of the cell · 2013Review
- Variations in protein-flavin hydrogen bonding in a light, oxygen, voltage domain produce non-Arrhenius kinetics of adduct decay.Biochemistry · 2011Article
- Tripping the light fantastic: blue-light photoreceptors as examples of environmentally modulated protein-protein interactions.Biochemistry · 2011Review
- Using light to control signaling cascades in live neurons.Current opinion in neurobiology · 2010Review
- Metabolic engineering, new antibiotics and biofilm viscoelasticity.Microbial biotechnology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Light control over enzyme function represents a novel and exciting field of biocatalysis research. Blue-light photoreceptors of the Light, Oxygen, Voltage (LOV) family have recently been investigated for their applicability as photoactive switches. We discuss here the primary photochemical events leading to light activation of LOV domains as well as the proposed signal propagation mechanism to the respective effector domain. Furthermore, we describe the construction of LOV fusions to different effector domains, namely a dihydrofolate reductase from Escherichia coli and a lipase from Bacillus subtilis. Both fusion partners retained functionality, and alteration of enzyme activity by light was also demonstrated. Hence, it appears that fusion of LOV photoreceptors to functional enzyme target sites via appropriate linker structures may represent a straightforward strategy to design light controllable biocatalysts.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.