ArticleMicrobial biotechnology2009
Dynamic of active microorganisms inhabiting a bioleaching industrial heap of low-grade copper sulfide ore monitored by real-time PCR and oligonucleotide prokaryotic acidophile microarray.
Article in Microbial biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Historic mine waste contains diverse microbial communities that reflect waste type and geochemistry.Applied and environmental microbiology · 2025Article
- Article
- Metal recovery from spent lithium-ion batteries via two-step bioleaching using adapted chemolithotrophs from an acidic mine pit lake.Frontiers in microbiology · 2024Article
- Hyperarid soil microbial community response to simulated rainfall.Frontiers in microbiology · 2023Article
- Article
- Identification and Unusual Properties of the Master Regulator FNR in the Extreme AcidophileFrontiers in microbiology · 2019Article
- Validation of Genetic Markers Associated to Oxygen Availability in Low-Grade Copper Bioleaching Systems: An Industrial Application.Frontiers in microbiology · 2019Article
- Pan-Genome Analysis Links the Hereditary Variation ofFrontiers in microbiology · 2018Article
- Living at the Frontiers of Life: Extremophiles in Chile and Their Potential for Bioremediation.Frontiers in microbiology · 2018Review
- Transmission in the Origins of Bacterial Diversity, From Ecotypes to Phyla.Microbiology spectrum · 2017Article
- Draft genome sequence ofStandards in genomic sciences · 2017Article
- Quantitative Monitoring of Microbial Species during Bioleaching of a Copper Concentrate.Frontiers in microbiology · 2016Article
- Community of thermoacidophilic and arsenic resistant microorganisms isolated from a deep profile of mine heaps.AMB Express · 2015Article
- Omics on bioleaching: current and future impacts.Applied microbiology and biotechnology · 2015Review
- Interaction networks for identifying coupled molecular processes in microbial communities.BioData mining · 2015Article
- Environmental, biogeographic, and biochemical patterns of archaea of the family Ferroplasmaceae.Applied and environmental microbiology · 2011Review
- Article
- The heat, drugs and knockout systems of microbial biotechnology.Microbial biotechnology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bioleaching of metal sulfide has developed into a very important industrial process and understanding the microbial dynamic is key to advancing commercial bioleaching operations. Here we report the first quantitative description of the dynamic of active communities in an industrial bioleaching heap. Acidithiobacillus ferrooxidans was the most abundant during the first part of the leaching cycle, while the abundance of Leptospirillum ferriphilum and Ferroplasma acidiphilum increased with age of the heap. Acidithiobacillus thiooxidans kept constant throughout the leaching cycle, and Firmicutes group showed a low and a patchy distribution in the heap. The Acidiphilium-like bacteria reached their highest abundance corresponding to the amount of autotrophs. The active microorganisms in the leaching system were determined using two RNA-based sensitive techniques. In most cases, the 16S rRNA copy numbers of At. ferrooxidans, L. ferriphilum, At. thiooxidans and F. acidiphilum, was concomitant with the DNA copy numbers, whereas Acidiphilium-like bacteria and some Firmicutes members did not show a clear correlation between 16S rRNA accumulation and DNA copy numbers. However, the prokaryotic acidophile microarray (PAM) analysis showed active members of Alphaproteobacteria in all samples and of Sulfobacillus genus in older ones. Also, new active groups such as Actinobacteria and Acidobacterium genus were detected by PAM. The results suggest that changes during the leaching cycle in chemical and physical conditions, such as pH and Fe(3+)/Fe(2+) ion rate, are primary factors shaping the microbial dynamic in the heap.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.