ArticleMicrobial biotechnology2009
Three independent signalling pathways repress motility in Pseudomonas fluorescens F113.
Article in Microbial biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 52 citations in OpenAlex.
- Genetic basis of biofilm formation and salt adaptation in the plant-beneficial strain Stutzerimonas stutzeri MJL19.Applied microbiology and biotechnology · 2025Article
- Multiple pathways impact the swarming motility ofMicrobiology spectrum · 2024Article
- Identifying the suite of genes central to swimming in the biocontrol bacteriumMicrobial genomics · 2024Article
- Multiple Pathways Impact Swarming Motility ofbioRxiv : the preprint server for biology · 2024Article
- GacA reduces virulence and increases competitiveness in planta in the tumorigenic olive pathogenFrontiers in plant science · 2024Article
- Adaption ofMicroorganisms · 2023Review
- Two-component system GacS/GacA, a global response regulator of bacterial physiological behaviors.Engineering microbiology · 2023Review
- Article
- The GacS/A-Rsm Pathway Positively Regulates Motility and Flagella Synthesis in Azotobacter vinelandii.Current microbiology · 2021Article
- Review
- Review
- Cyclic-di-GMP Regulates the Quorum-Sensing System and Biocontrol Activity of Pseudomonas fluorescens 2P24 through the RsmA and RsmE Proteins.Applied and environmental microbiology · 2020Article
- The diguanylate cyclase AdrA regulates flagellar biosynthesis in Pseudomonas fluorescens F113 through SadB.Scientific reports · 2019Article
- AmrZ is a major determinant of c-di-GMP levels in Pseudomonas fluorescens F113.Scientific reports · 2018Article
- SuhB Regulates the Motile-Sessile Switch inFrontiers in microbiology · 2017Article
- Article
- Bacterial rotary export ATPases are allosterically regulated by the nucleotide second messenger cyclic-di-GMP.The Journal of biological chemistry · 2015Article
- Chemotactic Motility of Pseudomonas fluorescens F113 under Aerobic and Denitrification Conditions.PloS one · 2015Article
- The Pseudomonas aeruginosa diguanylate cyclase GcbA, a homolog of P. fluorescens GcbA, promotes initial attachment to surfaces, but not biofilm formation, via regulation of motility.Journal of bacteriology · 2014Article
- The Sensor Kinase GacS Negatively Regulates Flagellar Formation and Motility in a Biocontrol Bacterium, Pseudomonas chlororaphis O6.The plant pathology journal · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Motility is one of the most important traits for rhizosphere colonization by pseudomonads. Despite this importance, motility is severely repressed in the rhizosphere-colonizing strain Pseudomonas fluorescens F113. This bacterium is unable to swarm under laboratory conditions and produce relatively small swimming haloes. However, phenotypic variants with the ability to swarm and producing swimming haloes up to 300% larger than the wild-type strain, arise during rhizosphere colonization. These variants harbour mutations in the genes encoding the GacA/GacS two-component system and in other genes. In order to identify genes and pathways implicated in motility repression, we have used generalized mutagenesis with transposons. Analysis of the mutants has shown that besides the Gac system, the Wsp system and the sadB gene, which have been previously implicated in cyclic di-GMP turnover, are implicated in motility repression: mutants in the gacS, sadB or wspR genes can swarm and produce swimming haloes larger than the wild-type strain. Epistasis analysis has shown that the pathways defined by each of these genes are independent, because double and triple mutants show an additive phenotype. Furthermore, GacS, SadB and WspR act at different levels. Expression of the fleQ gene, encoding the master regulator of flagella synthesis is higher in the gacS(-) and sadB(-) backgrounds than in the wild-type strain and this differential expression is reflected by a higher secretion of the flagellin protein FliC. Conversely, no differences in fleQ expression or FliC secretion were observed between the wild-type strain and the wspR(-) mutant.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.