ArticleBiochemistry2011
Site-directed alkylation studies with LacY provide evidence for the alternating access model of transport.
Article in Biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Iron Acquisition Systems of Gram-negative Bacterial Pathogens Define TonB-Dependent Pathways to Novel Antibiotics.Chemical reviews · 2021Review
- Solvent accessibility changes in a NaThe Journal of biological chemistry · 2020Article
- Plant glucose transporter structure and function.Pflugers Archiv : European journal of physiology · 2020Review
- Dynamic distinctions in the NaThe Journal of biological chemistry · 2017Article
- Thermodynamics of Nanobody Binding to Lactose Permease.Biochemistry · 2016Article
- Role of Conserved Gly-Gly Pairs on the Periplasmic Side of LacY.Biochemistry · 2016Article
- The Ec-NhaA antiporter switches from antagonistic to synergistic antiport upon a single point mutation.Scientific reports · 2016Article
- A chemiosmotic mechanism of symport.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Galactoside-binding site in LacY.Biochemistry · 2014Article
- Structure of sugar-bound LacY.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The periplasmic cavity of LacY mutant Cys154→Gly: how open is open?Biochemistry · 2013Article
- Trp replacements for tightly interacting Gly-Gly pairs in LacY stabilize an outward-facing conformation.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Mutagenesis and modeling to predict structural and functional characteristics of the Staphylococcus aureus MepA multidrug efflux pump.Journal of bacteriology · 2013Article
- Role of the irreplaceable residues in the LacY alternating access mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Evidence for an intermediate conformational state of LacY.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Insights to the evolution of Nucleobase-Ascorbate Transporters (NAT/NCS2 family) from the Cys-scanning analysis of xanthine permease XanQ.International journal of biochemistry and molecular biology · 2012Article
- Lactose permease and the alternating access mechanism.Biochemistry · 2011Article
- The role of transmembrane segment TM3 in the xanthine permease XanQ of Escherichia coli.The Journal of biological chemistry · 2011Article
- An early event in the transport mechanism of LacY protein: interaction between helices V and I.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In total, 59 single Cys-replacement mutants in helix VII and helix X of the lactose permease of Escherichia coli were subjected to site-directed fluorescence labeling in right-side-out membrane vesicles to complete the testing of Cys accessibility or reactivity. For both helices, accessibility/reactivity is relatively low at the level of the sugar-binding site where the helices are tightly packed. However, labeling of Cys substitutions in helix VII with tetramethylrhodamine-5-maleimide decreases from the middle toward the cytoplasmic end and increases toward the periplasmic end. Helix X is labeled mainly on the side facing the central hydrophilic cavity with relatively small or no changes in the presence of ligand. In contrast, sugar binding causes a significant increase in accessibility/reactivity at the periplasmic end of helix VII. When considered with similar findings from N-ethylmaleimide alkylation studies, the results confirm and extend support for the alternating access model.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.