ArticlePloS one2011
Differential conservation and divergence of fertility genes boule and dazl in the rainbow trout.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 62 citations in OpenAlex.
- Characterization of scp3 and Its Expression Dynamics Identifies the Critical Period for Meiotic Initiation in Female Turbot (Scophthalmus maximus).Journal of experimental zoology. Part B, Molecular and developmental evolution · 2026Article
- Article
- A simple and rapid method for fish sex identification based on recombinase-aided amplification and its use in Cynoglossus semilaevis.Scientific reports · 2021Article
- Article
- Transcriptome analysis of the gonads of olive flounder (Paralichthys olivaceus).Fish physiology and biochemistry · 2016Article
- Dazl is a critical player for primordial germ cell formation in medaka.Scientific reports · 2016Article
- A premeiotic function for boule in the planarian Schmidtea mediterranea.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Gonadal transcriptomic analysis and differentially expressed genes in the testis and ovary of the Pacific white shrimp (Litopenaeus vannamei).BMC genomics · 2015Article
- Medaka Oct4 is essential for pluripotency in blastula formation and ES cell derivation.Stem cell reviews and reports · 2015Article
- Article
- Prevalent RNA recognition motif duplication in the human genome.RNA (New York, N.Y.) · 2014Article
- Vasa identifies germ cells and critical stages of oogenesis in the Asian seabass.International journal of biological sciences · 2014Article
- Differential evolution of duplicated medakafish mitf genes.International journal of biological sciences · 2013Article
- Chromosome evolution and genome miniaturization in minifish.PloS one · 2012Article
- Boule-like genes regulate male and female gametogenesis in the flatworm Macrostomum lignano.Developmental biology · 2011Article
- Germ cell sex prior to meiosis in the rainbow trout.Protein & cell · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe genes boule and dazl are members of the DAZ (Deleted in Azoospermia) family encoding RNA binding proteins essential for germ cell development. Although dazl exhibits bisexual expression in mitotic and meiotic germ cells in diverse animals, boule shows unisexual meiotic expression in invertebrates and mammals but a bisexual mitotic and meiotic expression in medaka. How boule and dazl have evolved different expression patterns in diverse organisms has remained unknown. METHODOLOGY AND PRINCIPAL
findingsHere we chose the fish rainbow trout (Oncorhynchus mykiss) as a second lower vertebrate model to investigate the expression of boule and dazl. By molecular cloning and sequence comparison, we identified cDNAs encoding the trout Boule and Dazl proteins, which have a conserved RNA-recognition motif and a maximal similarity to their homologs. By RT-PCR analysis, adult RNA expression of trout boule and dazl is restricted to the gonads of both sexes. By chromogenic and two-color fluorescence in situ hybridization, we revealed bisexual and germline-specific expression of boule and dazl. We found that dazl displays conserved expression throughout gametogenesis and concentrates in the Balbinani's body of early oocytes and the chromatoid body of sperm. Surprisingly, boule exhibits mitotic and meiotic expression in the male but meiosis-specific expression in the female.
conclusionsOur data underscores differential conservation and divergence of DAZ family genes during vertebrate evolution. We propose a model in which the diversity of boule expression in sex and stage specificity might have resulted from selective loss or gain of its expression in one sex and mitotic germ cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.