ArticlePloS one2010
Natural variation of the amino-terminal glutamine-rich domain in Drosophila argonaute2 is not associated with developmental defects.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
- Recurrent expansion and rapid evolution of the Drosophilid RNAi pathway in testis.bioRxiv : the preprint server for biology · 2025Article
- Article
- Molecular mechanisms of biomolecular condensate formation in Drosophila melanogaster siRNA biogenesis.Nucleic acids research · 2025Article
- Condensin-mediated restriction of retrotransposable elements facilitates brain development in Drosophila melanogaster.Nature communications · 2024Article
- N-terminal region of Drosophila melanogaster Argonaute2 forms amyloid-like aggregates.BMC biology · 2023Article
- Transposable element landscapes in aging Drosophila.PLoS genetics · 2022Article
- Polyglutamine Repeats in Viruses.Molecular neurobiology · 2019Review
- Genome-Wide Association Study of Circadian Behavior in Drosophila melanogaster.Behavior genetics · 2019Article
- Review
- Article
- Repeated Duplication of Argonaute2 Is Associated with Strong Selection and Testis Specialization in Drosophila.Genetics · 2016Article
- Homology directed repair is unaffected by the absence of siRNAs in Drosophila melanogaster.Nucleic acids research · 2016Article
- Variation and Evolution in the Glutamine-Rich Repeat Region of Drosophila Argonaute-2.G3 (Bethesda, Md.) · 2016Article
- Duplication and Diversification of Dipteran Argonaute Genes, and the Evolutionary Divergence of Piwi and Aubergine.Genome biology and evolution · 2016Article
- A Polymorphism in the Processing Body Component Ge-1 Controls Resistance to a Naturally Occurring Rhabdovirus in Drosophila.PLoS pathogens · 2016Article
- As the fat flies: The dynamic lipid droplets of Drosophila embryos.Biochimica et biophysica acta · 2015Review
- Adaptive regulation of testis gene expression and control of male fertility by the Drosophila hairpin RNA pathway. [Corrected].Molecular cell · 2015Article
- Novel Drosophila viruses encode host-specific suppressors of RNAi.PLoS pathogens · 2014Article
- The Drosophila MAST kinase Drop out is required to initiate membrane compartmentalisation during cellularisation and regulates dynein-based transport.Development (Cambridge, England) · 2014Article
- The DNA virus Invertebrate iridescent virus 6 is a target of the Drosophila RNAi machinery.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
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Authors and funding
12 authors at 9 institutions in 4 countries.
Funding
Abstract
The Drosophila argonaute2 (ago2) gene plays a major role in siRNA mediated RNA silencing pathways. Unlike mammalian Argonaute proteins, the Drosophila protein has an unusual amino-terminal domain made up largely of multiple copies of glutamine-rich repeats (GRRs). We report here that the ago2 locus produces an alternative transcript that encodes a putative short isoform without this amino-terminal domain. Several ago2 mutations previously reported to be null alleles only abolish expression of the long, GRR-containing isoform. Analysis of drop out (dop) mutations had previously suggested that variations in GRR copy number result in defects in RNAi and embryonic development. However, we find that dop mutations genetically complement transcript-null alleles of ago2 and that ago2 alleles with variant GRR copy numbers support normal development. In addition, we show that the assembly of the central RNAi machinery, the RISC (RNA induced silencing complex), is unimpaired in embryos when GRR copy number is altered. In fact, we find that GRR copy number is highly variable in natural D. melanogaster populations as well as in laboratory strains. Finally, while many other insects share an extensive, glutamine-rich Ago2 amino-terminal domain, its primary sequence varies drastically between species. Our data indicate that GRR variation does not modulate an essential function of Ago2 and that the amino-terminal domain of Ago2 is subject to rapid evolution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.