Evidence map›Paper›PMID 21252943›Full record

ArticleEMBO reports2011

Purification and identification of endogenous polySUMO conjugates.

Roland Bruderer, Michael H Tatham, Anna Plechanovova, Ivan Matic, Amit K Garg, Ronald T Hay

Open access · bronzeAbstract read
In one paragraph

Article in EMBO reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers.

0numbers the graph read from it
0cells of the map it votes in
101citing papers in PubMed
10.9field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

101 citing papers in PubMed, 164 citations in OpenAlex.

  1. Assays to Study SUMOylation of RNA Helicase DHX9.Methods in molecular biology (Clifton, N.J.) · 2027
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41 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Roland BrudererWellcome Trust Centre for Gene Regulation and Expression, Dow Street, Dundee DD1 5EH, UK.
Michael H Tatham
Anna Plechanovova
Ivan Matic
Amit K Garg
Ronald T Hay
Wellcome Trust · GBUniversity of Dundee · GBCell and Gene Therapy Catapult · GB

Funding

Cancer Research UKWellcome Trust
6 · The paper itself

Abstract

The small ubiquitin-like modifier (SUMO) can undergo self-modification to form polymeric chains that have been implicated in cellular processes such as meiosis, genome maintenance and stress response. Investigations into the biological role of polymeric chains have been hampered by the absence of a protocol for the purification of proteins linked to SUMO chains. In this paper, we describe a rapid affinity purification procedure for the isolation of endogenous polySUMO-modified species that generates highly purified material suitable for individual protein studies and proteomic analysis. We use this approach to identify more than 300 putative polySUMO conjugates from cultured eukaryotic cells.

Indexed as

Amino Acid SequenceHeLa CellsHot TemperatureHumansMolecular Sequence DataNuclear ProteinsProtein BindingProtein Interaction Domains and MotifsProteomeStress, PhysiologicalSubcellular FractionsSumoylationTranscription FactorsNuclear ProteinsProteomeRNF4 protein, humanTranscription Factors

Identifiers

PMID21252943
PMCPMC3049431
OpenAlexW2154983385

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.