ArticleCells, tissues, organs2011
Engineered vascular tissue fabricated from aggregated smooth muscle cells.
Article in Cells, tissues, organs, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
33 citing papers in PubMed, 75 citations in OpenAlex.
- Cyclic stretch inhibits cell invasion in 3D scaffolds.bioRxiv : the preprint server for biology · 2026Article
- TNF-α and IL-1β but not IL-18 counteract TGF-β1 mediated stimulation of collagen accumulation and increased extracellular matrix strength and stiffness.Scientific reports · 2026Article
- Geometrically Tunable Scaffold-Free Muscle Bioconstructs for Treating Volumetric Muscle Loss.Advanced healthcare materials · 2026Article
- Endothelial Cells Increase Mesenchymal Stem Cell Differentiation in Scaffold-Free 3D Vascular Tissue.Tissue engineering. Part A · 2025Article
- TGFBR3 dependent mechanism of TGFB2 in smooth muscle cell differentiation and implications for TGFB2-related aortic aneurysm.Stem cells translational medicine · 2025Article
- An in vitro model to measure the strength and stiffness of the extracellular matrix synthesized de novo by human fibroblasts.In vitro models · 2025Article
- Integrated Experimental and Mathematical Exploration of Modular Tissue Cultures for Developmental Engineering.International journal of molecular sciences · 2024Article
- Temporal Enzymatic Treatment to Enhance the Remodeling of Multiple Cartilage Microtissues into a Structurally Organized Tissue.Advanced healthcare materials · 2024Article
- Small-Caliber Vascular Grafts Engineered from Decellularized Leaves and Cross-Linked Gelatin.Tissue engineering. Part A · 2023Article
- Quantifying Cell-Derived Changes in Collagen Synthesis, Alignment, and Mechanics in a 3D Connective Tissue Model.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- hiPSC Modeling of Lineage-Specific Smooth Muscle Cell Defects Caused byCirculation · 2021Article
- Xenogeneic-free generation of vascular smooth muscle cells from human induced pluripotent stem cells for vascular tissue engineering.Acta biomaterialia · 2021Article
- In Vitro Lineage-Specific Differentiation of Vascular Smooth Muscle Cells in Response to SMAD3 Deficiency: Implications for SMAD3-Related Thoracic Aortic Aneurysm.Arteriosclerosis, thrombosis, and vascular biology · 2020Article
- Circulating re-entrant waves promote maturation of hiPSC-derived cardiomyocytes in self-organized tissue ring.Communications biology · 2020Article
- A Method for High-Throughput Robotic Assembly of Three-Dimensional Vascular Tissue.Tissue engineering. Part A · 2019Article
- Assembly of Tissue-Engineered Blood Vessels with Spatially Controlled Heterogeneities.Tissue engineering. Part A · 2018Article
- A Modular Strategy to Engineer Complex Tissues and Organs.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Fabrication of Custom Agarose Wells for Cell Seeding and Tissue Ring Self-assembly Using 3D-Printed Molds.Journal of visualized experiments : JoVE · 2018Article
- Targeted Delivery of Bioactive Molecules for Vascular Intervention and Tissue Engineering.Frontiers in pharmacology · 2018Review
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Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The goal of this study was to develop a system to rapidly generate engineered tissue constructs from aggregated cells and cell-derived extracellular matrix (ECM) to enable evaluation of cell-derived tissue structure and function. Rat aortic smooth muscle cells seeded into annular agarose wells (2, 4 or 6 mm inside diameter) aggregated and formed thick tissue rings within 2 weeks of static culture (0.76 mm at 8 days; 0.94 mm at 14 days). Overall, cells appeared healthy and surrounded by ECM comprised of glycosoaminoglycans and collagen, although signs of necrosis were observed near the centers of the thickest rings. Tissue ring strength and stiffness values were superior to those reported for engineered tissue constructs cultured for comparable times. The strength (100-500 kPa) and modulus (0.5-2 MPa) of tissue rings increased with ring size and decreased with culture duration. Finally, tissue rings cultured for 7 days on silicone mandrels fused to form tubular constructs. Ring margins were visible after 7 days, but tubes were cohesive and mechanically stable, and histological examination confirmed fusion between ring subunits. This unique system provides a versatile new tool for optimization and functional assessment of cell-derived tissue, and a new approach to creating tissue-engineered vascular grafts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.