ArticleVirology journal2011
A reporter system for assaying influenza virus RNP functionality based on secreted Gaussia luciferase activity.
Article in Virology journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Homologous PB1 gene promotes the replication efficiency of avian influenza H7N4 candidate vaccine virus.Influenza and other respiratory viruses · 2022Article
- A Comprehensive Roadmap Towards the Generation of an Influenza B Reporter Assay Using a Single DNA Polymerase-Based Cloning of the Reporter RNA Construct.Frontiers in microbiology · 2022Article
- Development of a method for evaluating the mRNA transcription activity of influenza virus RNA-dependent RNA polymerase through real-time reverse transcription polymerase chain reaction.Virology journal · 2021Article
- Development and Characterization of a Reverse-Genetics System for Influenza D Virus.Journal of virology · 2019Article
- Screening for Novel Small-Molecule Inhibitors Targeting the Assembly of Influenza Virus Polymerase Complex by a Bimolecular Luminescence Complementation-Based Reporter System.Journal of virology · 2017Article
- A point mutation in the polymerase protein PB2 allows a reassortant H9N2 influenza isolate of wild-bird origin to replicate in human cells.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2016Article
- Adenovirus-mediated artificial MicroRNAs targeting matrix or nucleoprotein genes protect mice against lethal influenza virus challenge.Gene therapy · 2015Article
- Residues 41V and/or 210D in the NP protein enhance polymerase activities and potential replication of novel influenza (H7N9) viruses at low temperature.Virology journal · 2015Article
- Integrating computational modeling and functional assays to decipher the structure-function relationship of influenza virus PB1 protein.Scientific reports · 2014Article
- Genome rearrangement of influenza virus for anti-viral drug screening.Virus research · 2014Article
- Impact of the segment-specific region of the 3'-untranslated region of the influenza A virus PB1 segment on protein expression.Virus genes · 2013Article
- Structural and functional characterization of K339T substitution identified in the PB2 subunit cap-binding pocket of influenza A virus.The Journal of biological chemistry · 2013Article
- Organization of the influenza virus replication machinery.Science (New York, N.Y.) · 2012Article
- Mutations in polymerase genes enhanced the virulence of 2009 pandemic H1N1 influenza virus in mice.PloS one · 2012Article
- Development of recombinant rabies viruses vectors with Gaussia luciferase reporter based on Chinese vaccine strain CTN181.Virus research · 2011Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInfluenza A virus can infect a wide variety of animal species including humans, pigs, birds and other species. Viral ribonucleoprotein (vRNP) was involved in genome replication, transcription and host adaptation. Currently, firefly luciferase (Fluc) reporter system was used in vRNP functional assay. However, its limitation for the testing by virus infection resulted in an increased need for rapid, sensitive, and biosafe techniques. Here, an influenza A virus UTR-driven gene reporter for vRNP assay based on secreted Gaussia luciferase (Gluc) activity was evaluated.
resultsBy measuring Gluc levels in supernatants, reporter gene activity could be detected and quantitated after either reconstitution of influenza A virus polymerase complex or viral infection of 293T and A549 cells, respectively. As compared with Fluc reporter, Gluc-based reporter was heat-tolerant (65°C for 30 min) and produced 50-fold higher bioluminescent activity at 24 h posttransfection. Signals generated by Gluc reporter gene could be detected as early as 6 h post-infection and accumulated with time. Testing by viral infection, stronger signals were detected by Gluc reporter at a MOI of 0.001 than that of 1 and the effects of PB2-627K/E or amantadine on influenza vRNP activity were elucidated more effectively by the Gluc reporter system.
conclusionsThis approach provided a rapid, sensitive, and biosafe assay of influenza vRNP function, particularly for the highly pathogenic avian influenza viruses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.