ArticleThe Biochemical journal2011
Transcriptional regulation of the Zrg17 zinc transporter of the yeast secretory pathway.
Article in The Biochemical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Zinc homeostasis in Schizosaccharomyces pombe.Archives of microbiology · 2023Review
- Global Molecular Response ofJournal of fungi (Basel, Switzerland) · 2023Article
- Zinc at the Host-Fungus Interface: How to Uptake the Metal?Journal of fungi (Basel, Switzerland) · 2020Review
- Identification of the Genetic Requirements for Zinc Tolerance and Toxicity inG3 (Bethesda, Md.) · 2020Article
- Transcription factors and transporters in zinc homeostasis: lessons learned from fungi.Critical reviews in biochemistry and molecular biology · 2020Review
- The Loz1 transcription factor from Schizosaccharomyces pombe binds to Loz1 response elements and represses gene expression when zinc is in excess.Molecular microbiology · 2019Article
- Article
- Zinc transporters belonging to the Cation Diffusion Facilitator (CDF) family have complementary roles in transporting zinc out of the cytosol.PLoS genetics · 2018Article
- Zap1-dependent transcription from an alternative upstream promoter controls translation of RTC4 mRNA in zinc-deficient Saccharomyces cerevisiae.Molecular microbiology · 2017Article
- Effect of zinc deprivation on the lipid metabolism of budding yeast.Current genetics · 2017Review
- Identification of membrane proteome ofFuture science OA · 2017Article
- Regulation of cation balance in Saccharomyces cerevisiae.Genetics · 2013Review
- The ins and outs of algal metal transport.Biochimica et biophysica acta · 2012Review
- Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Msc2 and Zrg17 proteins of Saccharomyces cerevisiae are members of the cation diffusion facilitator family of zinc transporters. These proteins form heteromeric complexes that transport zinc into the ER (endoplasmic reticulum). Previous studies suggested that the ZRG17 gene is regulated in response to zinc status by the Zap1 transcription factor. Zap1 activates the expression of many genes in zinc-deficient cells. In the present study, we assessed whether ZRG17 is a direct Zap1 target gene. We showed that ZRG17 mRNA levels were elevated in zinc-limited cells in a Zap1-dependent manner and were also elevated in zinc-replete cells expressing a constitutively active allele of Zap1. Furthermore, Zrg17 protein levels correlated closely with mRNA levels. A candidate Zap1-binding site [ZRE (zinc-responsive element)] in the ZRG17 promoter was required for this induction. Using electrophoretic mobility-shift assays and chromatin immunoprecipitation, we demonstrated that Zap1 binds specifically to the ZRG17 ZRE both in vitro and in vivo. By using a chromosomal ZRG17 mutant with a non-functional ZRE, we found that Zap1 induction of ZRG17 is required for ER function as indicated by elevated ER stress under zinc-limited conditions. Together, these results establish that ZRG17 is a direct Zap1 target gene and its regulation has biological importance in maintaining ER function.
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