ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2011
Nuclei of chicken neurons in tissues and three-dimensional cell cultures are organized into distinct radial zones.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Why Do Some Vertebrates Have Microchromosomes?Cells · 2021Review
- Microchromosomes Exhibit Distinct Features of Vertebrate Chromosome Structure and Function with Underappreciated Ramifications for Genome Evolution.Molecular biology and evolution · 2021Article
- A new emu genome illuminates the evolution of genome configuration and nuclear architecture of avian chromosomes.Genome research · 2021Article
- FISH Going Meso-Scale: A Microscopic Search for Chromatin Domains.Frontiers in cell and developmental biology · 2021Review
- Heterochromatic regions in Japanese quail chromosomes: comprehensive molecular-cytogenetic characterization and 3D mapping in interphase nucleus.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2019Article
- 3D organization of chicken genome demonstrates evolutionary conservation of topologically associated domains and highlights unique architecture of erythrocytes' chromatin.Nucleic acids research · 2019Article
- Molecular cytogenetic characterization of repetitive sequences comprising centromeric heterochromatin in three Anseriformes species.PloS one · 2019Article
- Nuclear organization and 3D chromatin architecture in cognition and neuropsychiatric disorders.Molecular brain · 2016Review
- Nucleolus-like body of mouse oocytes contains lamin A and B and TRF2 but not actin and topo II.Molecular cytogenetics · 2016Article
- Chicken embryonic stem cells and primordial germ cells display different heterochromatic histone marks than their mammalian counterparts.Epigenetics & chromatin · 2016Article
- Three-dimensional architecture of tandem repeats in chicken interphase nucleus.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Article
- Interphase chromatin organisation in Arabidopsis nuclei: constraints versus randomness.Chromosoma · 2012Article
- Similar rye A and B chromosome organization in meristematic and differentiated interphase nuclei.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
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12 authors.
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Abstract
We used chicken retinospheroids (RS) to study the nuclear architecture of vertebrate cells in a three-dimensional (3D) cell culture system. The results showed that the different neuronal cell types of RS displayed an extreme form of radial nuclear organization. Chromatin was arranged into distinct radial zones which became already visible after DAPI staining. The distinct zones were enriched in different chromatin modifications and in different types of chromosomes. Active isoforms of RNA polymerase II were depleted in the outermost zone. Also chromocenters and nucleoli were radially aligned in the nuclear interior. The splicing factor SC35 was enriched at the central zone and did not show the typical speckled pattern of distribution. Evaluation of neuronal and non-neuronal chicken tissues showed that the highly ordered form of radial nuclear organization was also present in neuronal chicken tissues. Furthermore, the data revealed that the neuron-specific nuclear organization was remodeled when cells spread on a flat substrate. Monolayer cultures of a chicken cell line did not show this extreme form of radial organization. Rather, such monolayer cultures displayed features of nuclear organization which have been described before for many different types of monolayer cells. The finding that an extreme form radial nuclear organization, which has not been described before, is present in RS and tissues, but not in cells spread on a flat substrate, suggests that it would be important to complement studies on nuclear architecture performed with monolayer cells by studies on 3D cell culture systems and tissues.
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