ArticleImmunogenetics2011
Genomic screening by 454 pyrosequencing identifies a new human IGHV gene and sixteen other new IGHV allelic variants.
Article in Immunogenetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 63 citations in OpenAlex.
- IGHV allele similarity clustering improves genotype inference from adaptive immune receptor repertoire sequencing data.Nucleic acids research · 2023Article
- AIRR-C IG Reference Sets: curated sets of immunoglobulin heavy and light chain germline genes.Frontiers in immunology · 2023Article
- A BALB/c IGHV Reference Set, Defined by Haplotype Analysis of Long-Read VDJ-C Sequences From F1 (BALB/c x C57BL/6) Mice.Frontiers in immunology · 2022Article
- Novel Allele Detection Tool Benchmark and Application With Antibody Repertoire Sequencing Dataset.Frontiers in immunology · 2021Article
- Serine substitutions are linked to codon usage and differ for variable and conserved protein regions.Scientific reports · 2019Article
- Per-sample immunoglobulin germline inference from B cell receptor deep sequencing data.PLoS computational biology · 2019Article
- Identification of Subject-Specific Immunoglobulin Alleles From Expressed Repertoire Sequencing Data.Frontiers in immunology · 2019Article
- Deciphering evolution of immune recognition in antibodies.BMC structural biology · 2018Article
- The Bayesian optimist's guide to adaptive immune receptor repertoire analysis.Immunological reviews · 2018Review
- Characterization of the naive murine antibody repertoire using unamplified high-throughput sequencing.PloS one · 2018Article
- The Individual and Population Genetics of Antibody Immunity.Trends in immunology · 2017Review
- Association between a common immunoglobulin heavy chain allele and rheumatic heart disease risk in Oceania.Nature communications · 2017Article
- Production of individualized V gene databases reveals high levels of immunoglobulin genetic diversity.Nature communications · 2016Article
- Estimating Copy Number and Allelic Variation at the Immunoglobulin Heavy Chain Locus Using Short Reads.PLoS computational biology · 2016Article
- Evidence of perturbed germinal center dynamics, but preserved antibody diversity, in end-stage renal disease.Immunity, inflammation and disease · 2016Article
- Practical guidelines for B-cell receptor repertoire sequencing analysis.Genome medicine · 2015Review
- The mouse antibody heavy chain repertoire is germline-focused and highly variable between inbred strains.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Article
- Ability to develop broadly neutralizing HIV-1 antibodies is not restricted by the germline Ig gene repertoire.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Automated analysis of high-throughput B-cell sequencing data reveals a high frequency of novel immunoglobulin V gene segment alleles.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- The promise and challenge of high-throughput sequencing of the antibody repertoire.Nature biotechnology · 2014Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Complete and accurate knowledge of the genes and allelic variants of the human immunoglobulin gene loci is critical for studies of B cell repertoire development and somatic point mutation, but evidence from studies of VDJ rearrangements suggests that our knowledge of the available immunoglobulin gene repertoire is far from complete. The reported repertoire has changed little over the last 15 years. This is, in part, a consequence of the inefficiencies involved in searching for new members of large, multigenic gene families by cloning and sequencing. The advent of high-throughput sequencing provides a new avenue by which the germline repertoire can be explored. In this report, we describe pyrosequencing studies of the heavy chain IGHV1, IGHV3 and IGHV4 gene subgroups in ten Papua New Guineans. Thousands of 454 reads aligned with complete identity to 51 previously reported functional IGHV genes and allelic variants. A new gene, IGHV3-NL1*01, was identified, which differs from the nearest previously reported gene by 15 nucleotides. Sixteen new IGHV alleles were also identified, 15 of which varied from previously reported functional IGHV genes by between one and four nucleotides, while one sequence appears to be a functional variant of the pseudogene IGHV3-25. BLAST searches suggest that at least six of these new genes are carried within the relatively well-studied populations of North America, Europe or Asia. This study substantially expands the known immunoglobulin gene repertoire and demonstrates that genetic variation of immunoglobulin genes can now be efficiently explored in different human populations using high-throughput pyrosequencing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.