ArticleIntegrative biology : quantitative biosciences from nano to macro2011
Multifactorial optimization of endothelial cell growth using modular synthetic extracellular matrices.
Article in Integrative biology : quantitative biosciences from nano to macro, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
58 citing papers in PubMed, 116 citations in OpenAlex.
- RGD-functionalised self-assembling peptide hydrogel induces a proliferative profile in human osteoblasts in vitro.Journal of peptide science : an official publication of the European Peptide Society · 2025Article
- Design and applications of self-assembled polypeptide matrices in wound healing.Frontiers in bioengineering and biotechnology · 2025Review
- Engineered Protein Hydrogels as Biomimetic Cellular Scaffolds.Advanced materials (Deerfield Beach, Fla.) · 2024Review
- Advancements in self-assembling peptides: Bridging gaps in 3D cell culture and electronic device fabrication.Journal of biomaterials applications · 2024Review
- Synthetic extracellular matrices with function-encoding peptides.Nature reviews bioengineering · 2023Review
- Review
- Review
- Designer, injectable gels to prevent transplanted Schwann cell loss during spinal cord injury therapy.Science advances · 2020Article
- Immobilized RGD concentration and proteolytic degradation synergistically enhance vascular sprouting within hydrogel scaffolds of varying modulus.Journal of biomaterials science. Polymer edition · 2020Article
- Multifactorial Design of a Supramolecular Peptide Anti-IL-17 Vaccine Toward the Treatment of Psoriasis.Frontiers in immunology · 2020Article
- Combinatorial functionalization with bisurea-peptides and antifouling bisurea additives of a supramolecular elastomeric biomaterial.Journal of polymer science. Part B, Polymer physics · 2019Article
- Poly(ethylene glycol)-crosslinked gelatin hydrogel substrates with conjugated bioactive peptides influence endothelial cell behavior.Biomaterials · 2019Article
- Advances in immunotherapy delivery from implantable and injectable biomaterials.Acta biomaterialia · 2019Review
- Biomimetic modification of poly(vinyl alcohol): Encouraging endothelialization and preventing thrombosis with antiplatelet monotherapy.Acta biomaterialia · 2019Article
- Self-Assembling Peptide Gels for 3D Prostate Cancer Spheroid Culture.Macromolecular bioscience · 2019Article
- A 3D in vitro model of the dermoepidermal junction amenable to mechanical testing.Journal of biomedical materials research. Part A · 2018Article
- Review
- Nanoscale Peptide Self-assemblies Boost BCG-primed Cellular Immunity Against Mycobacterium tuberculosis.Scientific reports · 2018Article
- Engineering Approaches to Study Cellular Decision Making.Annual review of biomedical engineering · 2018Review
- Multicomponent peptide assemblies.Chemical Society reviews · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Extracellular matrices (ECMs) are complex materials, containing at least dozens of different macromolecules that are assembled together, thus complicating their optimization towards applications in 3D cell culture or tissue engineering. The natural complexity of ECMs has limited cell-matrix investigations predominantly to experiments where only one matrix component is adjusted at a time, making it difficult to uncover interactions between different matrix components or to efficiently determine optimal matrix compositions for specific desired biological responses. Here we have developed modular synthetic ECMs based on peptide self-assembly whose incorporation of multiple different peptide ligands can be adjusted. The peptides can co-assemble in a wide range of combinations to form hydrogels of uniform morphology and consistent mechanical properties, but with precisely varied mixtures of peptide ligands. The modularity of this system in turn enabled multi-factorial experimental designs for investigating interactions between these ligands and for determining a multi-peptide matrix formulation that maximized endothelial cell growth. In cultures of HUVECs, we observed a previously unknown antagonistic interaction between the laminin-derived peptide YIGSR and RGDS-mediated cell attachment and growth. We also identified an optimized combination of self-assembled peptides bearing the ligands RGDS and IKVAV that led to endothelial cell growth equivalent to that on native full-length fibronectin. Both of these findings would have been challenging to uncover using more traditional one-factor-at-a-time analyses.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.