ArticlemBio2011
Cyclic diguanylate signaling proteins control intracellular growth of Legionella pneumophila.
Article in mBio, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 60 citations in OpenAlex.
- The common HAQ STING variant impairs cGAS-dependent antibacterial responses and is associated with susceptibility to Legionnaires' disease in humans.PLoS pathogens · 2018Trial
- Small molecule communication ofMicrobiology and molecular biology reviews : MMBR · 2024Review
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- A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genes.eLife · 2023Article
- A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genes.bioRxiv : the preprint server for biology · 2023Article
- Rapid adaptations ofMicrobial genomics · 2023Article
- TheApplied and environmental microbiology · 2022Article
- Characterization of a Novel Regulator of Biofilm Formation in the PathogenBiomolecules · 2022Article
- Structural Conservation and Diversity of PilZ-Related Domains.Journal of bacteriology · 2020Article
- A Two-Component System That Modulates Cyclic di-GMP Metabolism Promotes Legionella pneumophila Differentiation and Viability in Low-Nutrient Conditions.Journal of bacteriology · 2019Article
- Factors Mediating Environmental Biofilm Formation byFrontiers in cellular and infection microbiology · 2018Review
- The Flagellar Regulon ofFrontiers in cellular and infection microbiology · 2017Review
- Phenotypic and Transcriptomic Responses ofFrontiers in microbiology · 2017Article
- Direct targeting of membrane fusion by SNARE mimicry: Convergent evolution of Legionella effectors.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Cyclic Di-GMP Regulates Multiple Cellular Functions in the Symbiotic Alphaproteobacterium Sinorhizobium meliloti.Journal of bacteriology · 2016Article
- Cyclic Di-GMP Regulates Type IV Pilus-Dependent Motility in Myxococcus xanthus.Journal of bacteriology · 2016Article
- Transcriptomic changes of Legionella pneumophila in water.BMC genomics · 2015Article
- Article
- Cyclic di-GMP-dependent signaling pathways in the pathogenic Firmicute Listeria monocytogenes.PLoS pathogens · 2014Article
- Three antagonistic cyclic di-GMP-catabolizing enzymes promote differential Dot/Icm effector delivery and intracellular survival at the early steps of Legionella pneumophila infection.Infection and immunity · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Proteins that metabolize or bind the nucleotide second messenger cyclic diguanylate regulate a wide variety of important processes in bacteria. These processes include motility, biofilm formation, cell division, differentiation, and virulence. The role of cyclic diguanylate signaling in the lifestyle of Legionella pneumophila, the causative agent of Legionnaires' disease, has not previously been examined. The L. pneumophila genome encodes 22 predicted proteins containing domains related to cyclic diguanylate synthesis, hydrolysis, and recognition. We refer to these genes as cdgS (cyclic diguanylate signaling) genes. Strains of L. pneumophila containing deletions of all individual cdgS genes were created and did not exhibit any observable growth defect in growth medium or inside host cells. However, when overexpressed, several cdgS genes strongly decreased the ability of L. pneumophila to grow inside host cells. Expression of these cdgS genes did not affect the Dot/Icm type IVB secretion system, the major determinant of intracellular growth in L. pneumophila. L. pneumophila strains overexpressing these cdgS genes were less cytotoxic to THP-1 macrophages than wild-type L. pneumophila but retained the ability to resist grazing by amoebae. In many cases, the intracellular-growth inhibition caused by cdgS gene overexpression was independent of diguanylate cyclase or phosphodiesterase activities. Expression of the cdgS genes in a Salmonella enterica serovar Enteritidis strain that lacks all diguanylate cyclase activity indicated that several cdgS genes encode potential cyclases. These results indicate that components of the cyclic diguanylate signaling pathway play an important role in regulating the ability of L. pneumophila to grow in host cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.