ArticlePloS one2011
Identification of lysine 37 of histone H2B as a novel site of methylation.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- DNA Repair in Nucleosomes: Insights from Histone Modifications and Mutants.International journal of molecular sciences · 2024Review
- Formaldehyde reacts with N-terminal proline residues to give bicyclic aminals.Communications chemistry · 2023Article
- Systematic genetic and proteomic screens during gametogenesis identify H2BK34 methylation as an evolutionary conserved meiotic mark.Epigenetics & chromatin · 2020Article
- Filter-Aided Sample Preparation Procedure for Mass Spectrometric Analysis of Plant Histones.Frontiers in plant science · 2018Article
- Choose Your Own Adventure: The Role of Histone Modifications in Yeast Cell Fate.Journal of molecular biology · 2017Review
- Engineered bromodomains to explore the acetylproteome.Proteomics · 2015Article
- Quantitative proteomic analysis of histone modifications.Chemical reviews · 2015Review
- A chromatin activity-based chemoproteomic approach reveals a transcriptional repressome for gene-specific silencing.Nature communications · 2014Article
- A highly conserved region within H2B is important for FACT to act on nucleosomes.Molecular and cellular biology · 2014Article
- Initial characterization of histone H3 serine 10 O-acetylation.Epigenetics · 2013Article
- Proteomic characterization of novel histone post-translational modifications.Epigenetics & chromatin · 2013Article
- Deciphering post-translational modification codes.FEBS letters · 2013Review
- DNA replication origin function is promoted by H3K4 di-methylation in Saccharomyces cerevisiae.Genetics · 2012Article
- H2B Tyr37 phosphorylation suppresses expression of replication-dependent core histone genes.Nature structural & molecular biology · 2012Article
- Ascending the nucleosome face: recognition and function of structured domains in the histone H2A-H2B dimer.Biochimica et biophysica acta · 2012Article
- Molecular mechanisms and potential functions of histone demethylases.Nature reviews. Molecular cell biology · 2012Review
- Histone H4 lysine 20 of Saccharomyces cerevisiae is monomethylated and functions in subtelomeric silencing.Biochemistry · 2011Article
- Breaking the histone code with quantitative mass spectrometry.Expert review of proteomics · 2011Review
- Quantitative proteomic approaches to studying histone modifications.Current chemical genomics · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Recent technological advancements have allowed for highly-sophisticated mass spectrometry-based studies of the histone code, which predicts that combinations of post-translational modifications (PTMs) on histone proteins result in defined biological outcomes mediated by effector proteins that recognize such marks. While significant progress has been made in the identification and characterization of histone PTMs, a full appreciation of the complexity of the histone code will require a complete understanding of all the modifications that putatively contribute to it. Here, using the top-down mass spectrometry approach for identifying PTMs on full-length histones, we report that lysine 37 of histone H2B is dimethylated in the budding yeast Saccharomyces cerevisiae. By generating a modification-specific antibody and yeast strains that harbor mutations in the putative site of methylation, we provide evidence that this mark exist in vivo. Importantly, we show that this lysine residue is highly conserved through evolution, and provide evidence that this methylation event also occurs in higher eukaryotes. By identifying a novel site of histone methylation, this study adds to our overall understanding of the complex number of histone modifications that contribute to chromatin function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.