ArticlePloS one2011
Histone variants and their post-translational modifications in primary human fat cells.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- Glutamate Methylation, a Novel Histone Mark in Diatoms: Mass Spectrometry Identification and Structural Characterization.Plant direct · 2025Article
- Conditional KnockoutInternational journal of molecular sciences · 2025Article
- Deficiency of histone variant macroH2A1.1 is associated with sexually dimorphic obesity in mice.Scientific reports · 2023Article
- Protein arginine methylation in transcription and epigenetic regulation.Frontiers in epigenetics and epigenomics · 2023Review
- The dark side of histones: genomic organization and role of oncohistones in cancer.Clinical epigenetics · 2021Review
- Histone MacroH2A1: A Chromatin Point of Intersection between Fasting, Senescence and Cellular Regeneration.Genes · 2017Review
- Characterization of Individual Histone Posttranslational Modifications and Their Combinatorial Patterns by Mass Spectrometry-Based Proteomics Strategies.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Extracting histones for the specific purpose of label-free MS.Proteomics · 2016Article
- Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis.Journal of visualized experiments : JoVE · 2016Article
- H3K36ac Is an Evolutionary Conserved Plant Histone Modification That Marks Active Genes.Plant physiology · 2016Article
- ETD Outperforms CID and HCD in the Analysis of the Ubiquitylated Proteome.Journal of the American Society for Mass Spectrometry · 2015Article
- Quantitative proteomic analysis of histone modifications.Chemical reviews · 2015Review
- Profiling post-translational modifications of histones in human monocyte-derived macrophages.Proteome science · 2015Article
- Article
- Mass spectrometry-based proteomics for the analysis of chromatin structure and dynamics.International journal of molecular sciences · 2013Article
- Peptide identification by tandem mass spectrometry with alternate fragmentation modes.Molecular & cellular proteomics : MCP · 2012Review
- Breaking the histone code with quantitative mass spectrometry.Expert review of proteomics · 2011Review
- Differential phosphorylation of ribosomal proteins in Arabidopsis thaliana plants during day and night.PloS one · 2011Article
- The role of Lamin A in cytoskeleton organization in colorectal cancer cells: a proteomic investigation.Nucleus (Austin, Tex.)Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epigenetic changes related to human disease cannot be fully addressed by studies of cells from cultures or from other mammals. We isolated human fat cells from subcutaneous abdominal fat tissue of female subjects and extracted histones from either purified nuclei or intact cells. Direct acid extraction of whole adipocytes was more efficient, yielding about 100 µg of protein with histone content of 60%-70% from 10 mL of fat cells. Differential proteolysis of the protein extracts by trypsin or ArgC-protease followed by nanoLC/MS/MS with alternating CID/ETD peptide sequencing identified 19 histone variants. Four variants were found at the protein level for the first time; particularly HIST2H4B was identified besides the only H4 isoform earlier known to be expressed in humans. Three of the found H2A potentially organize small nucleosomes in transcriptionally active chromatin, while two H2AFY variants inactivate X chromosome in female cells. HIST1H2BA and three of the identified H1 variants had earlier been described only as oocyte or testis specific histones. H2AFX and H2AFY revealed differential and variable N-terminal processing. Out of 78 histone modifications by acetylation/trimethylation, methylation, dimethylation, phosphorylation and ubiquitination, identified from six subjects, 68 were found for the first time. Only 23 of these modifications were detected in two or more subjects, while all the others were individual specific. The direct acid extraction of adipocytes allows for personal epigenetic analyses of human fat tissue, for profiling of histone modifications related to obesity, diabetes and metabolic syndrome, as well as for selection of individual medical treatments.
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