ArticleBiology of reproduction2011
Loss of methylation at H19 DMD is associated with biallelic expression and reduced development in cattle derived by somatic cell nuclear transfer.
Article in Biology of reproduction, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 50 citations in OpenAlex.
- Epigenetics in development, disease, and therapy: from mechanisms to clinical interventions.Science China. Life sciences · 2026Review
- Dynamic methylation pattern of H19DMR and KvDMR1 in bovine oocytes and preimplantation embryos.Journal of assisted reproduction and genetics · 2024Article
- Exogenous OCT4 and SOX2 Contribution to In Vitro Reprogramming in Cattle.Biomedicines · 2023Article
- Establishment of bovine expanded potential stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Catalytic inhibition of H3K9me2 writers disturbs epigenetic marks during bovine nuclear reprogramming.Scientific reports · 2020Article
- Imprinting disorder in donor cells is detrimental to the development of cloned embryos in pigs.Oncotarget · 2017Article
- Aberrant DNA methylation reprogramming in bovine SCNT preimplantation embryos.Scientific reports · 2016Article
- Transcriptomic Features of Bovine Blastocysts Derived by Somatic Cell Nuclear Transfer.G3 (Bethesda, Md.) · 2015Article
- Epigenetic consequences of artificial reproductive technologies to the bovine imprinted genes SNRPN, H19/IGF2, and IGF2R.Frontiers in genetics · 2015Article
- Trichostatin A rescues the disrupted imprinting induced by somatic cell nuclear transfer in pigs.PloS one · 2015Article
- Development to term of cloned cattle derived from donor cells treated with valproic acid.PloS one · 2014Article
- Article
- Uncoupled embryonic and extra-embryonic tissues compromise blastocyst development after somatic cell nuclear transfer.PloS one · 2012Article
- Generation of Induced Pluripotent Stem (iPS) Cells by Nuclear Reprogramming.Stem cells international · 2011Article
- A concise review on epigenetic regulation: insight into molecular mechanisms.International journal of molecular sciences · 2011Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Although cloning of mammals has been achieved successfully, the percentage of live offspring is very low because of reduced fetal size and fewer implantation sites. Recent studies have attributed such pathological conditions to abnormal reprogramming of the donor cell used for cloning. The inability of the oocyte to fully restore the differentiated status of a somatic cell to its pluripotent and undifferentiated state is normally evidenced by aberrant DNA methylation patterns established throughout the genome during development to blastocyst. These aberrant methylation patterns are associated with abnormal expression of imprinted genes, which among other genes are essential for normal embryo development and gestation. We hypothesized that embryo loss and low implantation rates in cattle derived by somatic cell nuclear transfer (SCNT) are caused by abnormal epigenetic reprogramming of imprinted genes. To verify our hypothesis, we analyzed the parental expression and the differentially methylated domain (DMD) methylation status of the H19 gene. Using a parental-specific analysis, we confirmed for the first time that H19 biallelic expression is tightly associated with a severe demethylation of the paternal H19 DMD in SCNT embryos, suggesting that these epigenetic anomalies to the H19 locus could be directly responsible for the reduced size and low implantation rates of cloned embryos in cattle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.