ArticleJournal of clinical microbiology2011
The rtxA toxin gene of Kingella kingae: a pertinent target for molecular diagnosis of osteoarticular infections.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 67 citations in OpenAlex.
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- The role of real-time PCR testing in the investigation of paediatric patients with community-onset osteomyelitis and septic arthritis.Irish journal of medical science · 2019Article
- A New Highly Sensitive and Specific Real-Time PCR Assay Targeting the Malate Dehydrogenase Gene of Kingella kingae and Application to 201 Pediatric Clinical Specimens.Journal of clinical microbiology · 2018Article
- Molecular Tests That Target the RTX Locus Do Not Distinguish between Kingella kingae and the Recently Described Kingella negevensis Species.Journal of clinical microbiology · 2017Article
- Pore forming activity of the potent RTX-toxin produced by pediatric pathogen Kingella kingae: Characterization and comparison to other RTX-family members.Biochimica et biophysica acta · 2015Article
- Kingella kingae: carriage, transmission, and disease.Clinical microbiology reviews · 2015Review
- Evaluation of a real-time PCR assay for simultaneous detection of Kingella kingae and Staphylococcus aureus from synovial fluid in suspected septic arthritis.Annals of laboratory medicine · 2014Article
- RTX toxin plays a key role in Kingella kingae virulence in an infant rat model.Infection and immunity · 2014Article
- Outbreaks of Kingella kingae infections in daycare facilities.Emerging infectious diseases · 2014Review
- Kingella kingae septic arthritis in children: recognising an elusive pathogen.Journal of children's orthopaedics · 2014Article
- Calcium binding properties of the Kingella kingae PilC1 and PilC2 proteins have differential effects on type IV pilus-mediated adherence and twitching motility.Journal of bacteriology · 2013Article
- Article
Corrections and comments
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Authors and funding
12 authors at 7 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Kingella kingae is an emerging osteoarticular pathogen in young children. Its isolation by traditional culture methods remains difficult, underscoring the need to implement other diagnostic methods for its detection and identification, such as nucleic acid amplification tests. Although the genome of this bacterium has not yet been sequenced, a toxin named RTX has been identified. The goal of this study was to develop sensitive, specific, and rapid molecular methods based on the rtxA toxin gene sequence to diagnose this infection. Two real-time PCR assays (SYBR green and TaqMan chemistries) targeting this gene are reported. Sensitivity and specificity were first evaluated successfully with 67 strains: 31 Kingella kingae isolates and 36 strains from other bacterial species. Then, 52 clinical specimens positive or negative by culture and/or PCR (16S rRNA and cpn60 genes) were tested with these assays. A nested PCR assay with subsequent sequencing was also developed to confirm the presence of Kingella kingae isolates in these clinical specimens. The results obtained demonstrate that these assays are accurate for the diagnosis of Kingella kingae infection.
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