ArticleJournal of the Royal Society, Interface2011
Collagen gel three-dimensional matrices combined with adhesive proteins stimulate neuronal differentiation of mesenchymal stem cells.
Article in Journal of the Royal Society, Interface, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Engineering 3D neural models of neurodegeneration: the Neural Microenvironment Design Code (NMDC), design principles, and emerging new approach methodologies.NAM journal · 2026Review
- Toward strategies to advance the conductive properties of hydrogel formulations for cardiac tissue engineering.iScience · 2025Review
- Three-Dimensional Cell Drawing Technique in Hydrogel Using Micro Injection System.Micromachines · 2022Article
- Drug delivery to the central nervous system.Nature reviews. Materials · 2022Article
- Protective Mechanism and Treatment of Neurogenesis in Cerebral Ischemia.Neurochemical research · 2020Review
- Generation of pancreatic β cells for treatment of diabetes: advances and challenges.Stem cell research & therapy · 2018Review
- Modular approach for resolving and mapping complex neural and other cellular structures and their associated deformation fields in three dimensions.Nature protocols · 2018Article
- 3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer.Journal of visualized experiments : JoVE · 2018Article
- Hydrogel-assisted neuroregeneration approaches towards brain injury therapy: A state-of-the-art review.Computational and structural biotechnology journal · 2018Review
- Effects of Type I Collagen Concentration in Hydrogel on the Growth and Phenotypic Expression of Rat Chondrocytes.Tissue engineering and regenerative medicine · 2017Article
- Signaling of extracellular matrices for tissue regeneration and therapeutics.Tissue engineering and regenerative medicine · 2016Review
- Knockout of the adp gene related with colonization in Bacillus nematocida B16 using customized transcription activator-like effectors nucleases.Microbial biotechnology · 2015Article
- Different forms of tenascin-C with tenascin-R regulate neural differentiation in bone marrow-derived human mesenchymal stem cells.Tissue engineering. Part A · 2014Article
- Introduction to cell-hydrogel mechanosensing.Interface focus · 2014Review
- Peripheral nerve repair in rats using composite hydrogel-filled aligned nanofiber conduits with incorporated nerve growth factor.Tissue engineering. Part A · 2013Article
- The effect of differentiation induction on FAK and Src activity in live HMSCs visualized by FRET.PloS one · 2013Article
- Novel porous scaffolds of poly(lactic acid) produced by phase-separation using room temperature ionic liquid and the assessments of biocompatibility.Journal of materials science. Materials in medicine · 2012Article
- A biomaterials approach to peripheral nerve regeneration: bridging the peripheral nerve gap and enhancing functional recovery.Journal of the Royal Society, Interface · 2012Review
- Differentiation of human neural progenitor cells in functionalized hydrogel matrices.BioResearch open access · 2012Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three-dimensional gel matrices provide specialized microenvironments that mimic native tissues and enable stem cells to grow and differentiate into specific cell types. Here, we show that collagen three-dimensional gel matrices prepared in combination with adhesive proteins, such as fibronectin (FN) and laminin (LN), provide significant cues to the differentiation into neuronal lineage of mesenchymal stem cells (MSCs) derived from rat bone marrow. When cultured within either a three-dimensional collagen gel alone or one containing either FN or LN, and free of nerve growth factor (NGF), the MSCs showed the development of numerous neurite outgrowths. These were, however, not readily observed in two-dimensional culture without the use of NGF. Immunofluorescence staining, western blot and fluorescence-activated cell sorting analyses demonstrated that a large population of cells was positive for NeuN and glial fibrillary acidic protein, which are specific to neuronal cells, when cultured in the three-dimensional collagen gel. The dependence of the neuronal differentiation of MSCs on the adhesive proteins containing three-dimensional gel matrices is considered to be closely related to focal adhesion kinase (FAK) activation through integrin receptor binding, as revealed by an experiment showing no neuronal outgrowth in the FAK-knockdown cells and stimulation of integrin β1 gene. The results provided herein suggest the potential role of three-dimensional collagen-based gel matrices combined with adhesive proteins in the neuronal differentiation of MSCs, even without the use of chemical differentiation factors. Furthermore, these findings suggest that three-dimensional gel matrices might be useful as nerve-regenerative scaffolds.
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