ArticleNucleic acids research2011
A piggyBac transposon-based mutagenesis system for the fission yeast Schizosaccharomyces pombe.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
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- Hijacking Transposable Elements for Saturation Mutagenesis in Fungi.Frontiers in fungal biology · 2021Review
- Genome Engineering of Yarrowia lipolytica with the PiggyBac Transposon System.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Comparing the utility of in vivo transposon mutagenesis approaches in yeast species to infer gene essentiality.Current genetics · 2020Article
- Atg38-Atg8 interaction in fission yeast establishes a positive feedback loop to promote autophagy.Autophagy · 2020Article
- Identification of Essential Genes and Fluconazole Susceptibility Genes inG3 (Bethesda, Md.) · 2020Article
- Basal transcription profiles of the rhamnose-inducible promoter PCurrent genetics · 2019Article
- Potential for Isolation of Immortalized Hepatocyte Cell Lines by Liver-DirectedStem cells international · 2019Review
- Candida albicans gains azole resistance by altering sphingolipid composition.Nature communications · 2018Article
- Vast potential for using the piggyBac transposon to engineer transgenic plants at specific genomic locations.Bioengineered · 2016Article
- Article
- A non-autonomous insect piggyBac transposable element is mobile in tobacco.Molecular genetics and genomics : MGG · 2014Article
- Mdb1, a fission yeast homolog of human MDC1, modulates DNA damage response and mitotic spindle function.PloS one · 2014Article
- A nucleolus-predominant piggyBac transposase, NP-mPB, mediates elevated transposition efficiency in mammalian cells.PloS one · 2014Article
- A rice Stowaway MITE for gene transfer in yeast.PloS one · 2013Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The TTAA-specific transposon piggyBac (PB), originally isolated from the cabbage looper moth, Trichoplusia ni, has been utilized as an insertional mutagenesis tool in various eukaryotic organisms. Here, we show that PB transposes in the fission yeast Schizosaccharomyces pombe and leaves almost no footprints. We developed a PB-based mutagenesis system for S. pombe by constructing a strain with a selectable transposon excision marker and an integrated transposase gene. PB transposition in this strain has low chromosomal distribution bias as shown by deep sequencing-based insertion site mapping. Using this system, we obtained loss-of-function alleles of klp5 and klp6, and a gain-of-function allele of dam1 from a screen for mutants resistant to the microtubule-destabilizing drug thiabendazole. From another screen for cdc25-22 suppressors, we obtained multiple alleles of wee1 as expected. The success of these two screens demonstrated the usefulness of this PB-mediated mutagenesis tool for fission yeast.
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