ArticleBiomaterials2011
Hydrogel network design using multifunctional macromers to coordinate tissue maturation in ovarian follicle culture.
Article in Biomaterials, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
82 citing papers in PubMed, 2 syntheses or guidelines pooled it, 151 citations in OpenAlex.
- Bioengineering trends in female reproduction: a systematic review.Human reproduction update · 2022Pooled it
- New advances in ovarian autotransplantation to restore fertility in cancer patients.Cancer metastasis reviews · 2015Pooled it
- Three-Dimensional Ovary Model to Improve and Study Murine Follicle Growth.Advanced healthcare materials · 2026Article
- Building a human pluripotent stem cell-based gonadal niche: improving in vitro systems with in vivo insights.Human reproduction update · 2025Review
- GelMA Core-Shell Microgel Preparation Based on a Droplet Microfluidic Device for Three-Dimensional Tumor Ball Culture and Its Drug Testing.Molecules (Basel, Switzerland) · 2025Article
- A matrix-free 3D in vitro follicle culture system in mice exhibits enhanced oocyte meiotic and developmental competence compared to hydrogel encapsulation.Molecular human reproduction · 2025Article
- Three-dimensional culture in a bioengineered matrix and somatic cell complementation to improve growth and survival of bovine preantral follicles.Journal of assisted reproduction and genetics · 2025Article
- Ovarian Mechanobiology: Understanding the Interplay Between Mechanics and Follicular Development.Cells · 2025Review
- Silk-Ovarioids: establishment and characterization of a human ovarian primary cell 3D-model system.Human reproduction open · 2025Article
- Building-Block Size Mediates Microporous Annealed Particle Hydrogel Tube Microenvironment Following Spinal Cord Injury.Advanced healthcare materials · 2024Article
- Three-Dimensionally Printed Agarose Micromold Supports Scaffold-Free Mouse Ex Vivo Follicle Growth, Ovulation, and Luteinization.Bioengineering (Basel, Switzerland) · 2024Article
- Extracellular matrix-templating fibrous hydrogels promote ovarian tissue remodeling and oocyte growth.Bioactive materials · 2024Article
- In Vitro Growth of Human Follicles: Current and Future Perspectives.International journal of molecular sciences · 2024Review
- Advances and challenges in regenerative therapies for abdominal aortic aneurysm.Frontiers in cardiovascular medicine · 2024Review
- Hydrolytic hydrogels tune mesenchymal stem cell persistence and immunomodulation for enhanced diabetic cutaneous wound healing.Biomaterials · 2023Article
- Creating a semi-opened micro-cavity ovary through sacrificial microspheres as anBioactive materials · 2023Article
- Novel Approaches Used in Ovarian Tissue Transplantation for Fertility Preservation: Focus on Tissue Engineering Approaches and Angiogenesis Capacity.Reproductive sciences (Thousand Oaks, Calif.) · 2023Review
- Recapitulating folliculogenesis and oogenesis outside the body: encapsulated in vitro follicle growth†.Biology of reproduction · 2023Article
- Roadmap on biomaterials for women's health.JPhys materials · 2023Article
- Current progress onFrontiers in cell and developmental biology · 2023Review
22 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Synthetic hydrogels with tunable properties are appealing for regenerative medicine. A critical limitation in hydrogel design at low solids concentration is the formation of defects, which increase gelation times and swelling, and reduce elasticity. Here, we report that trifunctional cross-linking peptides applied to 4-arm poly-(ethylene glycol) (PEG) hydrogels decreased swelling and gelation time relative to bi-functional crosslinkers. In contrast to bi-functional peptides, the third cross-linking site on the peptide created a branch point if an intramolecular cross-link formed, which prevented non-functional "dangling-ends" in the hydrogel network and enhanced the number of elastically active cross-links. The improved network formation enabled mouse ovarian follicle encapsulation and maturation in vitro. Hydrogels with bi-functional crosslinkers resulted in cellular dehydration, likely due to osmosis during the prolonged gelation. For trifunctional crosslinkers, the hydrogels supported a 17-fold volumetric expansion of the tissue during culture, with expansion dependent on the ability of the follicle to rearrange its microenvironment, which is controlled through the sensitivity of the cross-linking peptide to the proteolytic activity of plasmin. The improved network design enabled ovarian follicle culture in a completely synthetic system, and can advance fertility preservation technology for women facing premature infertility from anticancer therapies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.