ArticleMolecular bioSystems2011
Identification of an FHL1 protein complex containing ACTN1, ACTN4, and PDLIM1 using affinity purifications and MS-based protein-protein interaction analysis.
Article in Molecular bioSystems, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Deep muscle-proteomic analysis of freeze-dried human muscle biopsies reveals fiber type-specific adaptations to exercise training.Nature communications · 2021Trial
- Alms1 KO Rat: A New Model of Cardiometabolic Syndrome With Spontaneous Hypertension.Acta physiologica (Oxford, England) · 2026Article
- Transcriptome Analysis of Myocardial Ischemic-Hypoxic Injury in Rats and Hypoxic H9C2 Cells.ESC heart failure · 2024Article
- Molecular composition of skeletal muscle in infants and adults: a comparative proteomic and transcriptomic study.Scientific reports · 2024Article
- PDLIM1 interacts with HK2 to promote gastric cancer progression through enhancing the Warburg effect via Wnt/β-catenin signaling.Cell and tissue research · 2024Article
- The unexpected versatility of ALP/Enigma family proteins.Frontiers in cell and developmental biology · 2022Review
- PDLIM1: Structure, function and implication in cancer.Cell stress · 2021Review
- FHL1 Inhibits the Progression of Colorectal Cancer by Regulating the Wnt/β-Catenin Signaling Pathway.Journal of Cancer · 2021Article
- Autophagy protects cardiomyocytes from the myocardial ischaemia-reperfusion injury through the clearance of CLP36.Open biology · 2016Article
- Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function.Nature communications · 2015Article
- Transcriptional Response to Acute Thermal Exposure in Juvenile Chinook Salmon Determined by RNAseq.G3 (Bethesda, Md.) · 2015Article
- 199th ENMC international workshop: FHL1 related myopathies, June 7-9, 2013, Naarden, The Netherlands.Neuromuscular disorders : NMD · 2014Article
- HACE1-dependent protein degradation provides cardiac protection in response to haemodynamic stress.Nature communications · 2014Article
- Up-regulated FHL1 expression maybe involved in the prognosis of Hirschsprung's disease.International journal of medical sciences · 2014Article
- Design and formulation of functional pluripotent stem cell-derived cardiac microtissues.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Alp/Enigma family proteins cooperate in Z-disc formation and myofibril assembly.PLoS genetics · 2013Article
- Identification of an FHL1 protein complex containing gamma-actin and non-muscle myosin IIB by analysis of protein-protein interactions.PloS one · 2013Article
- α-Crystallin B prevents apoptosis after H2O2 exposure in mouse neonatal cardiomyocytes.American journal of physiology. Heart and circulatory physiology · 2012Article
- SIRPA is a specific cell-surface marker for isolating cardiomyocytes derived from human pluripotent stem cells.Nature biotechnology · 2011Article
- Four and a half LIM protein 1C (FHL1C): a binding partner for voltage-gated potassium channel K(v1.5).PloS one · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
introductionFour and a half LIM domains protein 1 (FHL1) is the most widely expressed member of the FHL family of proteins, consisting of four and a half highly conserved LIM domains. A multifunctional and integral role for FHL1 has been implicated in muscle development, structural maintenance, and signaling. To date, 27 FHL1 mutations have been identified that result in at least six different X-linked myopathies, with patients often presenting with cardiovascular complications. Since proteins assemble into dynamic complexes within the cell, FHL1 likely mediates its biological functions in conjunction with other proteins. Delineation of FHL1 interactions could provide insight into its regulatory functions.
methodsWe performed tandem affinity purification from human embryonic kidney 293 (HEK-293) cells to purify FHL1 and interacting proteins. To identify the potential interactors of FHL1 we performed a total of 9 different purifications from HEK-293 cells which included 3 experimental replicates for each biological condition: FHL1, tag control (DPYSL3), and negative control (empty vector). Purified samples were analyzed by liquid chromatography mass spectrometry (LC-MS). Potential interactors were then verified by immunoprecipitation from mouse heart ventricles and interactions visualized in adult cardiomyocytes using 3D fluorescence microscopy.
resultsWe identified a total of 310 different proteins from all 9 purifications and by applying stringent filtering criteria we eliminated all proteins found in any of the controls and only allowed those that were detected in two or more bait purification. We identified 34 high confidence potential binding partners of FHL1. We then showed that FHL1 exists as part of a complex that binds with PDLIM1, GSN and ACTN1.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.