ArticleInvestigative ophthalmology & visual science2011
In vivo high-frequency, contrast-enhanced ultrasonography of uveal melanoma in mice: imaging features and histopathologic correlations.
Article in Investigative ophthalmology & visual science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Comparative analysis of ultrasonic elastosonography and contrast-enhanced ultrasonography in the diagnosis of benign and malignant intraocular tumors.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2023Article
- Non-Invasive Imaging and Scoring of Peritoneal Metastases in Small Preclinical Animal Models Using Ultrasound: A Preliminary Trial.Biomedicines · 2022Article
- Animal Models of Uveal Melanoma.Annals of eye science · 2022Article
- Mouse models of uveal melanoma: Strengths, weaknesses, and future directions.Pigment cell & melanoma research · 2020Review
- Clinical utility, dose determination, and safety of ocular contrast-enhanced ultrasonography in horses: A pilot study.Veterinary ophthalmology · 2020Article
- Quantitative, noninvasive assessment of intra- and extraocular perfusion by contrast-enhanced ultrasonography and its clinical applicability in healthy dogs.Veterinary ophthalmology · 2019Article
- Tetrac Delayed the Onset of Ocular Melanoma in an Orthotopic Mouse Model.Frontiers in endocrinology · 2018Article
- Low dose angiostatic treatment counteracts radiotherapy-induced tumor perfusion and enhances the anti-tumor effect.Oncotarget · 2016Article
- The Toll-like receptor 5 agonist entolimod suppresses hepatic metastases in a murine model of ocular melanoma via an NK cell-dependent mechanism.Oncotarget · 2016Article
- Animal Models of Uveal Melanoma: Methods, Applicability, and Limitations.BioMed research international · 2016Review
- Animal Eye Models for Uveal Melanoma.Ocular oncology and pathology · 2015Review
- Acoustic characterization of contrast-to-tissue ratio and axial resolution for dual-frequency contrast-specific acoustic angiography imaging.IEEE transactions on ultrasonics, ferroelectrics, and frequency control · 2014Article
- Characterizing EPR-mediated passive drug targeting using contrast-enhanced functional ultrasound imaging.Journal of controlled release : official journal of the Controlled Release Society · 2014Article
- In vivo high-frequency contrast-enhanced ultrasonography of choroidal melanoma in rabbits: imaging features and histopathologic correlations.The British journal of ophthalmology · 2013Article
- Modeling human choroidal melanoma xenograft growth in immunocompromised rodents to assess treatment efficacy.Investigative ophthalmology & visual science · 2012Article
- Combined Radiofrequency Ablation and Double Anti-Angiogenic Protein Therapy to Increase Coagulation Efficacy: An Experimental Study in a Murine Renal Carcinoma Model.Korean journal of radiologyArticle
- Ultrasonographic Assessment of Intrinsic Vascularity in Choroidal Nevus, Suspicious Choroidal Nevus, and Choroidal Melanoma.Ophthalmology scienceArticle
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
Abstract
purposeTo evaluate the usefulness of in vivo imaging of uveal melanoma in mice using high-frequency contrast-enhanced ultrasound (HF-CE-US) with 2D or 3D modes and to correlate the sonographic findings with histopathologic characteristics.
methodsFourteen 12-week-old C57BL6 mice were inoculated into their right eyes with aliquots of 5 × 10(5)/2.5 μL B16LS9 melanoma cells and were randomly assigned to either of two groups. At 7 days after inoculation, tumor-bearing eyes in group 1 (n = 8) were imaged using HF-CE-US to determine the 2D tumor size and relative blood volume; eyes in group 2 (n = 6) were imaged by 3D microbubble contrast-enhanced ultrasound, and the tumor volume was determined. Histologic tumor burden was quantified in enucleated eyes by image processing software, and microvascular density was determined by counting von Willebrand factor-positive vascular channels. Ultrasound images were evaluated and compared with histopathologic findings.
resultsUsing HF-CE-US, melanomas were visualized as relatively hyperechoic regions. The intraobserver variability of sonographic measurements was 9.65% ± 7.89%, and the coefficient of variation for multiple measurements was 7.33% ± 5.71%. The correlation coefficient of sonographic volume or size and histologic area was 0.71 (P = 0.11) and 0.79 (P = 0.32). The relative blood volume within the tumor demonstrated sonographically correlated significantly with histologic tumor vascularity (r = 0.83; P < 0.001).
conclusionsThere was a positive linear correlation between sonographic tumor measurements and histologic tumor burden in the mouse ocular melanoma model. Contrast-enhanced intensity corresponded with microvascular density and blood volume. HF-CE-US is a real-time, noninvasive, reliable method for in vivo evaluation of experimental intraocular melanoma tumor area and relative blood volume.
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