ArticleNucleic acids research2011
A polar barrier to transcription can be circumvented by remodeler-induced nucleosome translocation.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 32 citations in OpenAlex.
- DNA bendability regulates transcription factor binding to nucleosomes.Nature structural & molecular biology · 2025Article
- Histone tetrasome dynamics affects chromatin transcription.Nucleic acids research · 2025Article
- How does CHD4 slide nucleosomes?Biochemical Society transactions · 2024Review
- Collaboration through chromatin: motors of transcription and chromatin structure.Journal of molecular biology · 2021Review
- Cockayne syndrome B protein acts as an ATP-dependent processivity factor that helps RNA polymerase II overcome nucleosome barriers.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Training-free measures based on algorithmic probability identify high nucleosome occupancy in DNA sequences.Nucleic acids research · 2019Article
- Structural insights into the ability of nucleoplasmin to assemble and chaperone histone octamers for DNA deposition.Scientific reports · 2019Article
- Time-resolved analysis of transcription through chromatin.Methods (San Diego, Calif.) · 2019Article
- Biochemical methods to characterize RNA polymerase II elongation complexes.Methods (San Diego, Calif.) · 2019Article
- High precision FRET studies reveal reversible transitions in nucleosomes between microseconds and minutes.Nature communications · 2018Article
- Transcription and Remodeling Produce Asymmetrically Unwrapped Nucleosomal Intermediates.Molecular cell · 2017Article
- Nucleosomal signatures impose nucleosome positioning in coding and noncoding sequences in the genome.Genome research · 2016Article
- Nucleosomal Barrier to Transcription: Structural Determinants and Changes in Chromatin Structure.Biochemistry & molecular biology journal · 2016Article
- Structural analysis of nucleosomal barrier to transcription.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Experimental analysis of hFACT action during Pol II transcription in vitro.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Nucleosome-positioning sequence repeats impact chromatin silencing in yeast minichromosomes.Genetics · 2014Article
- Histone chaperone FACT action during transcription through chromatin by RNA polymerase II.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Mechanism of transcription through a nucleosome by RNA polymerase II.Biochimica et biophysica acta · 2013Review
- Structural Analysis of the Key Intermediate Formed during Transcription through a Nucleosome.Trends in cell & molecular biology · 2013Article
- Perfect and imperfect nucleosome positioning in yeast.Biochimica et biophysica acta · 2012Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Many eukaryotic genes are regulated at the level of transcript elongation. Nucleosomes are likely targets for this regulation. Previously, we have shown that nucleosomes formed on very strong positioning sequences (601 and 603), present a high, orientation-dependent barrier to transcription by RNA polymerase II in vitro. The existence of this polar barrier correlates with the interaction of a 16-bp polar barrier signal (PBS) with the promoter-distal histone H3-H4 dimer. Here, we show that the polar barrier is relieved by ISW2, an ATP-dependent chromatin remodeler, which translocates the nucleosome over a short distance, such that the PBS no longer interacts with the distal H3-H4 dimer, although it remains within the nucleosome. In vivo, insertion of the 603 positioning sequence into the yeast CUP1 gene results in a modest reduction in transcription, but this reduction is orientation-independent, indicating that the polar barrier can be circumvented. However, the 603-nucleosome is present at the expected position in only a small fraction of cells. Thus, the polar barrier is probably non-functional in vivo because the nucleosome is not positioned appropriately, presumably due to nucleosome sliding activities. We suggest that interactions between PBSs and chromatin remodelers might have significant regulatory potential.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.