ArticleBiophysical journal2011
Vinculin activation is necessary for complete talin binding.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 47 citations in OpenAlex.
- Mechanomedicine: Present state and future promise.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Molecular dynamics simulations reveal how vinculin refolds partially unfolded talin rod helices to stabilize them against mechanical force.PLoS computational biology · 2024Article
- Advances in modeling cellular mechanical perceptions and responses via the membrane-cytoskeleton-nucleus machinery.Mechanobiology in medicine · 2024Review
- On the Value of In Vitro Cell Systems for Mechanobiology from the Perspective of Yes-Associated Protein/Transcriptional Co-Activator with a PDZ-Binding Motif and Focal Adhesion Kinase and Their Involvement in Wound Healing, Cancer, Aging, and Senescence.International journal of molecular sciences · 2023Review
- Allosteric activation of vinculin by talin.Nature communications · 2023Article
- Durotaxis: the mechanical control of directed cell migration.The FEBS journal · 2022Review
- The Pertinent Role of Cell and Matrix Mechanics in Cell Adhesion and Migration.Frontiers in cell and developmental biology · 2021Article
- Kindlin Assists Talin to Promote Integrin Activation.Biophysical journal · 2020Article
- When Stiffness Matters: Mechanosensing in Heart Development and Disease.Frontiers in cell and developmental biology · 2020Review
- Kindlin Is Mechanosensitive: Force-Induced Conformational Switch Mediates Cross-Talk among Integrins.Biophysical journal · 2019Article
- Vinculin and metavinculin exhibit distinct effects on focal adhesion properties, cell migration, and mechanotransduction.PloS one · 2019Article
- Molecular Simulations Suggest a Force-Dependent Mechanism of Vinculin Activation.Biophysical journal · 2017Article
- Vinculin in cell-cell and cell-matrix adhesions.Cellular and molecular life sciences : CMLS · 2017Review
- Single and collective cell migration: the mechanics of adhesions.Molecular biology of the cell · 2017Article
- Mechanosensitive Conformation of Vinculin Regulates Its Binding to MAPK1.Biophysical journal · 2017Article
- Vinculin association with actin cytoskeleton is necessary for stiffness-dependent regulation of vinculin behavior.PloS one · 2017Article
- Article
- Vinculin phosphorylation at residues Y100 and Y1065 is required for cellular force transmission.Journal of cell science · 2015Article
- Structural Determinants of the Mechanical Stability of α-Catenin.The Journal of biological chemistry · 2015Article
- The talin dimer structure orientation is mechanically regulated.Biophysical journal · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Focal adhesions are critical to a number of cellular processes that involve mechanotransduction and mechanical interaction with the cellular environment. The growth and strengthening of these focal adhesions is dependent on the interaction between talin and vinculin. This study investigates said interaction and how vinculin activation influences it. Using molecular dynamics, the interaction between talin's vinculin binding site (VBS) and vinculin's domain 1 (D1) is simulated both before and after vinculin activation. The simulations of VBS binding to vinculin before activation suggest the proximity of the vinculin tail to D1 prevents helical movement in D1 and thus prevents binding of VBS. In contrast, interaction of VBS with activated vinculin shows the possibility of complete VBS insertion into D1. In the simulations of both activated and autoinhibited vinculin where VBS fails to fully bind, VBS demonstrates significant hydrophobic interaction with surface residues in D1. These interactions link VBS to D1 even without its proper insertion into the hydrophobic core. Together these simulations suggest VBS binds to vinculin with the following mechanism: 1), VBS links to D1 via surface hydrophobic interactions; 2), vinculin undergoes activation and D1 is moved away from the vinculin tail; 3), helices in D1 undergo conformational change to allow VBS binding; and 4), VBS inserts itself into the hydrophobic core of D1.
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