ArticleBMC microbiology2011
A combined approach for comparative exoproteome analysis of Corynebacterium pseudotuberculosis.
Article in BMC microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 68 citations in OpenAlex.
- Proteomics of Toxigenic Corynebacteria.Proteomes · 2023Review
- Genetic Engineering and Biosynthesis Technology: Keys to Unlocking the Chains of Phage Therapy.Viruses · 2023Review
- Multi-Omics ofProteomes · 2022Article
- A journey through thePeerJ · 2021Article
- Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains ofFrontiers in veterinary science · 2021Article
- In vivo and in vitro expression of five genes involved in Corynebacterium pseudotuberculosis virulence.AMB Express · 2018Article
- Surface and Extracellular Proteome of the Emerging PathogenProteomes · 2018Article
- Identification of membrane-associated proteins with pathogenic potential expressed by Corynebacterium pseudotuberculosis grown in animal serum.BMC research notes · 2018Article
- Label-free quantitative proteomics of Corynebacterium pseudotuberculosis isolates reveals differences between Biovars ovis and equi strains.BMC genomics · 2017Article
- A shift in the virulence potential of Corynebacterium pseudotuberculosis biovar ovis after passage in a murine host demonstrated through comparative proteomics.BMC microbiology · 2017Article
- Quantitative Proteomic Analysis Reveals Changes in the BenchmarkFrontiers in cellular and infection microbiology · 2017Article
- Humoral and cellular immune responses in mice against secreted and somatic antigens from a Corynebacterium pseudotuberculosis attenuated strain: Immune response against a C. pseudotuberculosis strain.BMC veterinary research · 2016Article
- Penicillin binding protein 3 of Staphylococcus aureus NCTC 8325-4 binds and activates human plasminogen.BMC research notes · 2016Article
- Mass spectrometry based proteomics for absolute quantification of proteins from tumor cells.Methods (San Diego, Calif.) · 2015Article
- How are the non-classically secreted bacterial proteins released into the extracellular milieu?Current microbiology · 2013Review
- Differential Exoproteome analysis of two corynebacterium pseudotuberculosis biovar ovis strains isolated from goat (1002) and sheep (C231).Current microbiology · 2013Article
- Chromobacterium violaceum: important insights for virulence and biotechnological potential by exoproteomic studies.Current microbiology · 2013Article
- Distribution of PLD and FagA, B, C and D genes in Corynebacterium pseudotuberculosis isolates from sheep and goats with caseus lymphadenitis.Genetics and molecular biology · 2013Article
- Ion Torrent-based transcriptional assessment of a Corynebacterium pseudotuberculosis equi strain reveals denaturing high-performance liquid chromatography a promising rRNA depletion method.Microbial biotechnology · 2013Article
- Cell envelope of corynebacteria: structure and influence on pathogenicity.ISRN microbiology · 2013Article
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Authors and funding
18 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBacterial exported proteins represent key components of the host-pathogen interplay. Hence, we sought to implement a combined approach for characterizing the entire exoproteome of the pathogenic bacterium Corynebacterium pseudotuberculosis, the etiological agent of caseous lymphadenitis (CLA) in sheep and goats.
resultsAn optimized protocol of three-phase partitioning (TPP) was used to obtain the C. pseudotuberculosis exoproteins, and a newly introduced method of data-independent MS acquisition (LC-MSE) was employed for protein identification and label-free quantification. Additionally, the recently developed tool SurfG+ was used for in silico prediction of sub-cellular localization of the identified proteins. In total, 93 different extracellular proteins of C. pseudotuberculosis were identified with high confidence by this strategy; 44 proteins were commonly identified in two different strains, isolated from distinct hosts, then composing a core C. pseudotuberculosis exoproteome. Analysis with the SurfG+ tool showed that more than 75% (70/93) of the identified proteins could be predicted as containing signals for active exportation. Moreover, evidence could be found for probable non-classical export of most of the remaining proteins.
conclusionsComparative analyses of the exoproteomes of two C. pseudotuberculosis strains, in addition to comparison with other experimentally determined corynebacterial exoproteomes, were helpful to gain novel insights into the contribution of the exported proteins in the virulence of this bacterium. The results presented here compose the most comprehensive coverage of the exoproteome of a corynebacterial species so far.
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