ArticleThe EMBO journal2011
The Arabidopsis CUL4-DDB1 complex interacts with MSI1 and is required to maintain MEDEA parental imprinting.
Article in The EMBO journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 79 citations in OpenAlex.
- The Ubiquitin-Proteasome System in Flowering Plant Reproduction: Mechanisms, Functional Diversity, and Regulatory Networks.Plants (Basel, Switzerland) · 2026Review
- Pre-meiotic H1.1 degradation is essential for Arabidopsis gametogenesis.The EMBO journal · 2026Article
- Spatiotemporal Transcriptomic Atlas Reveals the Regulatory Mechanisms Underlying Early Inflorescence Development and Sex Differentiation in Spinach.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Nuclear lamina component KAKU4 regulates chromatin states and transcriptional regulation in the Arabidopsis genome.BMC biology · 2024Article
- Cloning and expression analysis of DnMSI1 gene in orchid speciesPlant signaling & behavior · 2022Article
- UBP12 and UBP13 deubiquitinases destabilize the CRY2 blue light receptor to regulate Arabidopsis growth.Current biology : CB · 2022Article
- Progressive chromatin silencing of ABA biosynthesis genes permits seed germination in Arabidopsis.The Plant cell · 2022Article
- The Importance of Networking: Plant Polycomb Repressive Complex 2 and Its Interactors.Epigenomes · 2022Review
- Genome-wide Identification, Expression, and Functional Analysis ofFrontiers in genetics · 2022Article
- The Roles of Cullins E3 Ubiquitin Ligases in the Lipid Biosynthesis of the Green MicroalgaeInternational journal of molecular sciences · 2021Article
- Roles of plant retinoblastoma protein: cell cycle and beyond.The EMBO journal · 2020Review
- Genome-wide identification of WD40 genes reveals a functional diversification of COP1-like genes in Rosaceae.Plant molecular biology · 2020Article
- Conservation of centromeric histone 3 interaction partners in plants.Journal of experimental botany · 2020Article
- Editor Profile: Pascal Genschik.The Plant cell · 2020Article
- Article
- Arabidopsis CRL4 Complexes: Surveying Chromatin States and Gene Expression.Frontiers in plant science · 2019Review
- MicroRNA-133b inhibits cell proliferation and promotes apoptosis by targeting cullin 4B in esophageal squamous cell carcinoma.Experimental and therapeutic medicine · 2018Article
- Article
- Genomic Imprinting in the Endosperm Is Systematically Perturbed in Abortive Hybrid Tomato Seeds.Molecular biology and evolution · 2016Article
- Epigenetic regulation of Kcna3-encoding Kv1.3 potassium channel by cereblon contributes to regulation of CD4+ T-cell activation.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein ubiquitylation regulates a broad variety of biological processes in all eukaryotes. Recent work identified a novel class of cullin-containing ubiquitin ligases (E3s) composed of CUL4, DDB1, and one WD40 protein, believed to act as a substrate receptor. Strikingly, CUL4-based E3 ligases (CRL4s) have important functions at the chromatin level, including responses to DNA damage in metazoans and plants and, in fission yeast, in heterochromatin silencing. Among putative CRL4 receptors we identified MULTICOPY SUPPRESSOR OF IRA1 (MSI1), which belongs to an evolutionary conserved protein family. MSI1-like proteins contribute to different protein complexes, including the epigenetic regulatory Polycomb repressive complex 2 (PRC2). Here, we provide evidence that Arabidopsis MSI1 physically interacts with DDB1A and is part of a multimeric protein complex including CUL4. CUL4 and DDB1 loss-of-function lead to embryo lethality. Interestingly, as in fis class mutants, cul4 mutants exhibit autonomous endosperm initiation and loss of parental imprinting of MEDEA, a target gene of the Arabidopsis PRC2 complex. In addition, after pollination both MEDEA transcript and protein accumulate in a cul4 mutant background. Overall, our work provides the first evidence of a physical and functional link between a CRL4 E3 ligase and a PRC2 complex, thus indicating a novel role of ubiquitylation in the repression of gene expression.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.