ArticleThe Plant cell2011
Glutathione-indole-3-acetonitrile is required for camalexin biosynthesis in Arabidopsis thaliana.
Article in The Plant cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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57 citing papers in PubMed, 126 citations in OpenAlex.
- HDA6 represses the histone acetylation modification and expression of WRKY18/40 to inhibit camalexin biosynthesis in Arabidopsis.Plant cell reports · 2026Article
- Epigenetically regulated transcriptional repressor, BASIC PENTACYSTEINE1 (BPC1) acts together with polycomb repressive complex 2 (PRC2) to suppress camalexin biosynthesis in Arabidopsis.Plant molecular biology · 2026Article
- A retrotransposon insertion upstream of Arabidopsis thaliana CRK8 receptor-like kinase modulates a trade-off between pathogen defense and salt tolerance.The Plant journal : for cell and molecular biology · 2025Article
- Cysteine Signalling in Plant Pathogen Response.Plant, cell & environment · 2025Article
- Sophisticated crosstalk of tryptophan-derived metabolites in plant stress responses.Plant communications · 2025Review
- Plasticity of the Arabidopsis leaf lipidome and proteome in response to pathogen infection and heat stress.Plant physiology · 2025Article
- Non-Targeted Metabolome Analysis with Low-Dose Selenate-TreatedPlants (Basel, Switzerland) · 2025Article
- Dual Roles of Reducing Systems in Protein Persulfidation and Depersulfidation.Antioxidants (Basel, Switzerland) · 2025Review
- Overlooked and misunderstood: can glutathione conjugates be clues to understanding plant glutathione transferases?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Enhanced disease resistance againstJournal of pesticide science · 2024Article
- Glutathione Transferase Photoaffinity Labeling Displays GST Induction by Safeners and Pathogen Infection.Plant & cell physiology · 2024Article
- Syntaxin of plants71 plays essential roles in plant development and stress response via regulating pH homeostasis.Frontiers in plant science · 2023Article
- Epipolythiodioxopiperazine-Based Natural Products: Building Blocks, Biosynthesis and Biological Activities.Chembiochem : a European journal of chemical biology · 2022Review
- Multilayered synergistic regulation of phytoalexin biosynthesis by ethylene, jasmonate, and MAPK signaling pathways in Arabidopsis.The Plant cell · 2022Article
- Glutathione contributes to plant defence against parasitic cyst nematodes.Molecular plant pathology · 2022Article
- Camalexin accumulation as a component of plant immunity during interactions with pathogens and beneficial microbes.Planta · 2022Review
- New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action.Frontiers in plant science · 2022Article
- Reconstruction of lateral root formation through single-cell RNA sequencing reveals order of tissue initiation.Molecular plant · 2021Article
- The Versatile Roles of Sulfur-Containing Biomolecules in Plant Defense-A Road to Disease Resistance.Plants (Basel, Switzerland) · 2020Review
- Differential Phosphorylation of the Transcription Factor WRKY33 by the Protein Kinases CPK5/CPK6 and MPK3/MPK6 Cooperatively Regulates Camalexin Biosynthesis in Arabidopsis.The Plant cell · 2020Article
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9 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Camalexin, a major phytoalexin in Arabidopsis thaliana, consists of an indole ring and a thiazole ring. The indole ring is produced from Trp, which is converted to indole-3-acetonitrile (IAN) by CYP79B2/CYP79B3 and CYP71A13. Conversion of Cys(IAN) to dihydrocamalexic acid and subsequently to camalexin is catalyzed by CYP71B15. Recent studies proposed that Cys derivative, not Cys itself, is the precursor of the thiazole ring that conjugates with IAN. The nature of the Cys derivative and how it conjugates to IAN and subsequently forms Cys(IAN) remain obscure. We found that protein accumulation of multiple glutathione S-transferases (GSTs), elevation of GST activity, and consumption of glutathione (GSH) coincided with camalexin production. GSTF6 overexpression increased and GSTF6-knockout reduced camalexin production. Arabidopsis GSTF6 expressed in yeast cells catalyzed GSH(IAN) formation. GSH(IAN), (IAN)CysGly, and γGluCys(IAN) were determined to be intermediates within the camalexin biosynthetic pathway. Inhibitor treatments and mutant analyses revealed the involvement of γ-glutamyl transpeptidases (GGTs) and phytochelatin synthase (PCS) in the catabolism of GSH(IAN). The expression of GSTF6, GGT1, GGT2, and PCS1 was coordinately upregulated during camalexin biosynthesis. These results suggest that GSH is the Cys derivative used during camalexin biosynthesis, that the conjugation of GSH with IAN is catalyzed by GSTF6, and that GGTs and PCS are involved in camalexin biosynthesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.