ArticleEukaryotic cell2011
Depletion of mitochondrial acyl carrier protein in bloodstream-form Trypanosoma brucei causes a kinetoplast segregation defect.
Article in Eukaryotic cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- A DNA polymerization-independent role for mitochondrial DNA polymerase I-like protein C in African trypanosomes.Journal of cell science · 2020Article
- Kinetoplast Division Factors in a Trypanosome.Trends in parasitology · 2019Review
- Molecular model of the mitochondrial genome segregation machinery inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- TAC102 Is a Novel Component of the Mitochondrial Genome Segregation Machinery in Trypanosomes.PLoS pathogens · 2016Article
- PNT1 Is a C11 Cysteine Peptidase Essential for Replication of the Trypanosome Kinetoplast.The Journal of biological chemistry · 2016Article
- The mitochondrial complex I of trypanosomatids--an overview of current knowledge.Journal of bioenergetics and biomembranes · 2014Review
- Mitochondrial and nucleolar localization of cysteine desulfurase Nfs and the scaffold protein Isu in Trypanosoma brucei.Eukaryotic cell · 2014Article
- Lipid synthesis in protozoan parasites: a comparison between kinetoplastids and apicomplexans.Progress in lipid research · 2013Review
- Trypanosome Letm1 protein is essential for mitochondrial potassium homeostasis.The Journal of biological chemistry · 2013Article
- Mitochondrial shape and function in trypanosomes requires the outer membrane protein, TbLOK1.Molecular microbiology · 2013Article
- Biochemistry and evolution of anaerobic energy metabolism in eukaryotes.Microbiology and molecular biology reviews : MMBR · 2012Review
- Enigmatic presence of mitochondrial complex I in Trypanosoma brucei bloodstream forms.Eukaryotic cell · 2012Article
- 25 years of African trypanosome research: From description to molecular dissection and new drug discovery.Molecular and biochemical parasitologyReview
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Like other eukaryotes, trypanosomes have an essential type II fatty acid synthase in their mitochondrion. We have investigated the function of this synthase in bloodstream-form parasites by studying the effect of a conditional knockout of acyl carrier protein (ACP), a key player in this fatty acid synthase pathway. We found that ACP depletion not only caused small changes in cellular phospholipids but also, surprisingly, caused changes in the kinetoplast. This structure, which contains the mitochondrial genome in the form of a giant network of several thousand interlocked DNA rings (kinetoplast DNA [kDNA]), became larger in some cells and smaller or absent in others. We observed the same pattern in isolated networks viewed by either fluorescence or electron microscopy. We found that the changes in kDNA size were not due to the disruption of replication but, instead, to a defect in segregation. kDNA segregation is mediated by the tripartite attachment complex (TAC), and we hypothesize that one of the TAC components, a differentiated region of the mitochondrial double membrane, has an altered phospholipid composition when ACP is depleted. We further speculate that this compositional change affects TAC function, and thus kDNA segregation.
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