ArticleHuman molecular genetics2011
Germline competency of parthenogenetic embryonic stem cells from immature oocytes of adult mouse ovary.
Article in Human molecular genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 15 citations in OpenAlex.
- Generation of developmentally competent oocytes and fertile mice from parthenogenetic embryonic stem cells.Protein & cell · 2021Article
- Study on the Function and Mechanism of Lin28B in the Formation of Chicken Primordial Germ Cells.Animals : an open access journal from MDPI · 2020Article
- Canonical MicroRNA Activity Facilitates but May Be Dispensable for Transcription Factor-Mediated Reprogramming.Stem cell reports · 2015Article
- Derivation of embryonic stem cells from Kunming mice IVF blastocyst in feeder- and serum-free condition.In vitro cellular & developmental biology. Animal · 2015Article
- Derivation of embryonic stem cell lines from parthenogenetically developing rat blastocysts.Stem cells and development · 2014Article
- Telomere elongation in parthenogenetic stem cells.Protein & cell · 2014Article
- Germline potential of parthenogenetic haploid mouse embryonic stem cells.Development (Cambridge, England) · 2012Article
- Quantitative proteomics analysis of parthenogenetically induced pluripotent stem cells.Protein & cell · 2011Article
Corrections and comments
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Authors and funding
18 authors at 6 institutions in 3 countries.
Funding
Abstract
Parthenogenetic embryonic stem cells (pESCs) have been generated in several mammalian species from parthenogenetic embryos that would otherwise die around mid-gestation. However, previous reports suggest that pESCs derived from in vivo ovulated (IVO) mature oocytes show limited pluripotency, as evidenced by low chimera production, high tissue preference and especially deficiency in germline competence, a critical test for genetic integrity and pluripotency of ESCs. Here, we report efficient generation of germline-competent pESC lines (named as IVM pESCs) from parthenogenetic embryos developed from immature oocytes of adult mouse ovaries following in vitro maturation (IVM) and artificial activation. In contrast, pESCs derived from IVO oocytes show defective germline competence, consistent with previous reports. Further, IVM pESCs resemble more ESCs from fertilized embryos (fESCs) than do IVO pESCs on genome-wide DNA methylation and global protein profiles. In addition, IVM pESCs express higher levels of Blimp1, Lin28 and Stella, relative to fESCs, and in their embryoid bodies following differentiation. This may indicate differences in differentiation potentially to the germline. The mechanisms for acquisition of pluripotency and germline competency of IVM pESCs from immature oocytes remain to be determined.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.