ArticleGenome integrity2011
Single- and double-stranded DNA binding proteins act in concert to conserve a telomeric DNA core sequence.
Article in Genome integrity, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Subtelomeric elements provide stability to short telomeres in telomerase-negative cells of the budding yeast Naumovozyma castellii.Current genetics · 2025Article
- The telomeric 5' end nucleotide is regulated in the budding yeast Naumovozyma castellii.Nucleic acids research · 2022Article
- PredPSD: A Gradient Tree Boosting Approach for Single-Stranded and Double-Stranded DNA Binding Protein Prediction.Molecules (Basel, Switzerland) · 2019Article
- Either Rap1 or Cdc13 can protect telomeric single-stranded 3' overhangs from degradation in vitro.Scientific reports · 2019Article
- The mechanisms of K. lactis Cdc13 in telomere DNA-binding and telomerase regulation.DNA repair · 2018Article
- Rap1 and Cdc13 have complementary roles in preventing exonucleolytic degradation of telomere 5' ends.Scientific reports · 2017Article
- Analysis and prediction of single-stranded and double-stranded DNA binding proteins based on protein sequences.BMC bioinformatics · 2017Article
- Double-stranded telomeric DNA binding proteins: Diversity matters.Cell cycle (Georgetown, Tex.) · 2017Review
- Evolution of Telomeres in Schizosaccharomyces pombe and Its Possible Relationship to the Diversification of Telomere Binding Proteins.PloS one · 2016Article
- Combinatorial recognition of a complex telomere repeat sequence by the Candida parapsilosis Cdc13AB heterodimer.Nucleic acids research · 2015Article
- Telomere DNA recognition in Saccharomycotina yeast: potential lessons for the co-evolution of ssDNA and dsDNA-binding proteins and their target sites.Frontiers in genetics · 2015Review
- Analysis and classification of DNA-binding sites in single-stranded and double-stranded DNA-binding proteins using protein information.IET systems biology · 2014Article
- Identification of single-stranded and double-stranded DNA binding proteins based on protein structure.BMC bioinformatics · 2014Article
- Telomerase-dependent generation of 70-nt-long telomeric single-stranded 3' overhangs in yeast.Nucleic acids research · 2013Article
- Rap1 binds single-stranded DNA at telomeric double- and single-stranded junctions and competes with Cdc13 protein.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTelomeres are protective cap structures at the ends of the linear eukaryotic chromosomes, which provide stability to the genome by shielding from degradation and chromosome fusions. The cap consists of telomere-specific proteins binding to the respective single- and double-stranded parts of the telomeric sequence. In addition to the nucleation of the chromatin structure the telomere-binding proteins are involved in the regulation of the telomere length. However, the telomeric sequences are highly diverged among yeast species. During the evolution this high rate of divergency presents a challenge for the sequence recognition of the telomere-binding proteins.
resultsWe found that the Saccharomyces castellii protein Rap1, a negative regulator of telomere length, binds a 12-mer minimal binding site (MBS) within the double-stranded telomeric DNA. The sequence specificity is dependent on the interaction with two 5 nucleotide motifs, having a 6 nucleotide centre-to-centre spacing. The isolated DNA-binding domain binds the same MBS and retains the same motif binding characteristics as the full-length Rap1 protein. However, it shows some deviations in the degree of sequence-specific dependence in some nucleotide positions. Intriguingly, the positions of most importance for the sequence-specific binding of the full-length Rap1 protein coincide with 3 of the 4 nucleotides utilized by the 3' overhang binding protein Cdc13. These nucleotides are very well conserved within the otherwise highly divergent telomeric sequences of yeasts.
conclusionsRap1 and Cdc13 are two very distinct types of DNA-binding proteins with highly separate functions. They interact with the double-stranded vs. the single-stranded telomeric DNA via significantly different types of DNA-binding domain structures. However, we show that they are dependent on coinciding nucleotide positions for their sequence-specific binding to telomeric sequences. Thus, we conclude that during the molecular evolution they act together to preserve a core sequence of the telomeric DNA.
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Registered trials
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