ArticleCellular reprogramming2011
SAF-A has a role in transcriptional regulation of Oct4 in ES cells through promoter binding.
Article in Cellular reprogramming, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- Regulation of DNA damage response by RNA/DNA-binding proteins: Implications for neurological disorders and aging.Ageing research reviews · 2024Review
- Biological functions and clinic significance of SAF‑A (Review).Biomedical reports · 2024Review
- Neurodevelopmental deficits and cell-type-specific transcriptomic perturbations in a mouse model of HNRNPU haploinsufficiency.PLoS genetics · 2023Article
- Article
- BAF complex-mediated chromatin relaxation is required for establishment of X chromosome inactivation.Nature communications · 2022Article
- Global Profiling of the Lysine Crotonylome in Different Pluripotent States.Genomics, proteomics & bioinformatics · 2021Article
- Tumorigenic effects of TLX overexpression in HEK 293T cells.Cancer reports (Hoboken, N.J.) · 2019Article
- HnRNPA1 interacts with G-quadruplex in the TRA2B promoter and stimulates its transcription in human colon cancer cells.Scientific reports · 2019Article
- Computational analysis of single-cell transcriptomics data elucidates the stabilization of Oct4 expression in the E3.25 mouse preimplantation embryo.Scientific reports · 2019Article
- The long non-coding RNA Snhg3 is essential for mouse embryonic stem cell self-renewal and pluripotency.Stem cell research & therapy · 2019Article
- HRPU-2, a Homolog of Mammalian hnRNP U, Regulates Synaptic Transmission by Controlling the Expression of SLO-2 Potassium Channel inThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- A novel long intergenic noncoding RNA indispensable for the cleavage of mouse two-cell embryos.EMBO reports · 2016Article
- Topological organization of multichromosomal regions by the long intergenic noncoding RNA Firre.Nature structural & molecular biology · 2014Article
- A comparison of pluripotency and differentiation status of four mesenchymal adult stem cells.Molecular biology reports · 2013Article
- Functions of heterogeneous nuclear ribonucleoproteins in stem cell potency and differentiation.BioMed research international · 2013Review
- SAF-A forms a complex with BRG1 and both components are required for RNA polymerase II mediated transcription.PloS one · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methodologies to reprogram somatic cells into patient-specific pluripotent cells, which could potentially be used in personalized drug discovery and cell replacement therapies, are currently under development. Oct4 activation is essential for successful reprogramming and pluripotency of embryonic stem (ES) cells, albeit molecular details of Oct4 activation are not completely understood. Here we report that endogenous SAF-A is involved in regulation of Oct4 expression, binds the Oct4 proximal promoter in ES cells, and dissociates from the promoter upon early differentiation induced by LIF withdrawal. Depletion of SAF-A decreases Oct4 expression even in the presence of LIF, and results in an increase of the mesodermal marker Brachyury. The overexpression of wild-type human SAF-A rescues the mouse knock-down phenotype and results in increased Oct4 level. We also demonstrate that endogenous SAF-A interacts with the C-terminal domain (CTD) of endogenous RNA polymerase II and that the interaction is independent of CTD phosphorylation and mRNA. Moreover, we show that SAF-A exist in complexes with transcription factors Sox2 and Oct4 as well as STAT3 in ES cells. The number of endogenous SAF-A:Oct4 and SAF-A:Sox2 complexes decreases upon LIF depletion. These discoveries allow us to propose a model for activation of Oct4 transcription.
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