ArticleEvidence-based complementary and alternative medicine : eCAM2011
Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers): Intervention in the Bcl-2-Mediated Apoptotic Pathway.
Article in Evidence-based complementary and alternative medicine : eCAM, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 57 citations in OpenAlex.
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- RETRACTION: Antiapoptotic and Antioxidant Properties ofEvidence-based complementary and alternative medicine : eCAM · 2025Article
- Review
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- Nano-liposomal encapsulation ofFood science & nutrition · 2022Article
- A New Strategy to Investigate the Efficacy Markers Underlying the Medicinal Potentials ofFrontiers in pharmacology · 2021Article
- Genotype selection for phytochemical content and pharmacological activities in ethanol extracts of fifteen types of Orthosiphon aristatus (Blume) Miq. leaves using chemometric analysis.Scientific reports · 2020Article
- Article
- Ethanolic Extract ofFrontiers in pharmacology · 2019Article
- Orthosiphon stamineus Extracts Inhibits Proliferation and Induces Apoptosis in Uterine Fibroid CellsAsian Pacific journal of cancer prevention : APJCP · 2018Article
- Southeast Asian Medicinal Plants as a Potential Source of Antituberculosis Agent.Evidence-based complementary and alternative medicine : eCAM · 2017Review
- Phytochemical Screening and Acute Oral Toxicity Study of Java Tea Leaf Extracts.BioMed research international · 2015Article
- Effects of a Proprietary Standardized Orthosiphon stamineus Ethanolic Leaf Extract on Enhancing Memory in Sprague Dawley Rats Possibly via Blockade of Adenosine A 2A Receptors.Evidence-based complementary and alternative medicine : eCAM · 2015Article
- Preparation and characterization of nano liposomes of Orthosiphon stamineus ethanolic extract in soybean phospholipids.BMC biotechnology · 2014Article
- Discovery of potential anti-infectives against Staphylococcus aureus using a Caenorhabditis elegans infection model.BMC complementary and alternative medicine · 2014Article
- Potent antioxidative and UVB protective effect of water extract of Eclipta prostrata L.TheScientificWorldJournal · 2014Article
- Acute toxicity and gastroprotective role of M. pruriens in ethanol-induced gastric mucosal injuries in rats.BioMed research international · 2013Article
- Acceleration of pro-caspase-3 maturation and cell migration inhibition in human breast cancer cells by phytoconstituents of Rheum emodi rhizome extracts.EXCLI journal · 2013Article
- In vivo antioxidant and antiulcer activity of Parkia speciosa ethanolic leaf extract against ethanol-induced gastric ulcer in rats.PloS one · 2013Article
Corrections and comments
- Retracted
Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antiapoptotic and antioxidant activities of aqueous-methanolic extract (CAME) of Orthosiphonstamineus Benth(OS), and its hexane (HF), chloroform (CF), n-butanol (NBF), ethyl acetate (EAF) and water (WF) fractions were investigated. Antioxidant properties were evaluated using the assays of Folin-Ciocalteu, aluminiumtrichloride, β-carotene bleaching and DPPH. The role of OS against hydrogen peroxide induced apoptosis on MDA-M231 epithelial cells was examined using MTT assay, phase contrast microscope, colorimetric assay of caspase-3, western blot and quantitative real-time PCR. Results showed that EAF showed the highest total phenolic content followed by CAME, NBF, WF, CF and HF, respectively. Flavonoid content was in the order of the CF > EAF > HF > CAME > NBF > WF. The IC(50) values on DPPH assay for different extract/fractions were 126.2 ± 23, 31.25 ± 1.2, 15.25 ± 2.3, 13.56 ± 1.9, 23.0 ± 3.2, and 16.66 ± 1.5 μg/ml for HF, CF, EAF, NBF, WF and CAME, respectively. OSreduced the oxidation of β-carotene by hydroperoxides. Cell death was dose-dependently inhibited by pretreatment with OS. Caspase-3 and distinct morphological features suggest the anti-apoptotic activities of OS. This plant not only increased the expression of Bcl-2, but also decreased Bax expression, and ultimately reduced H(2)O(2)-induced apoptosis. The current results showed that phenolics may provide health and nutritional benefits.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.