ArticleThe Korean journal of parasitology2010
Multiplex PCR detection of waterborne intestinal protozoa: microsporidia, Cyclospora, and Cryptosporidium.
Article in The Korean journal of parasitology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Use of DNA nanosensors based on upconverting nanoparticles for detection of Nosema bombycis by fluorescence resonance energy transfer.Folia microbiologica · 2022Article
- First Identification and Genotyping ofPathogens (Basel, Switzerland) · 2021Article
- Microfluidic multi-angle laser scattering system for rapid and label-free detection of waterborne parasites.Biomedical optics express · 2018Article
- Opportunistic and non-opportunistic intestinal parasites in HIV/ AIDS patients in relation to their clinical and epidemiological status in a specialized medical service in Goiás, Brazil.Revista do Instituto de Medicina Tropical de Sao Paulo · 2018Article
- Detection Methodologies for Pathogen and Toxins: A Review.Sensors (Basel, Switzerland) · 2017Review
- Detection of Intestinal Pathogens in River, Shore, and Drinking Water in Lima, Peru.Journal of genomics · 2017Article
- Detection and Molecular Characterization of Cryptosporidium spp. from Wild Rodents and Insectivores in South Korea.The Korean journal of parasitology · 2015Article
- Detection of Nosema bombycis by FTA cards and loop-mediated isothermal amplification (LAMP).Current microbiology · 2014Article
- Molecular testing for clinical diagnosis and epidemiological investigations of intestinal parasitic infections.Clinical microbiology reviews · 2014Review
- Epidemiological characteristics of the first water-borne outbreak of cryptosporidiosis in Seoul, Korea.Journal of Korean medical science · 2013Article
- Zinc PVA versus potassium dichromate for preservation of microsporidian spores of human origin.Parasitology research · 2012Article
- Microsporidiosis: not just in AIDS patients.Current opinion in infectious diseases · 2011Review
Corrections and comments
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Authors and funding
6 authors.
Funding
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Abstract
Recently, emerging waterborne protozoa, such as microsporidia, Cyclospora, and Cryptosporidium, have become a challenge to human health worldwide. Rapid, simple, and economical detection methods for these major waterborne protozoa in environmental and clinical samples are necessary to control infection and improve public health. In the present study, we developed a multiplex PCR test that is able to detect all these 3 major waterborne protozoa at the same time. Detection limits of the multiplex PCR method ranged from 10(1) to 10(2) oocysts or spores. The primers for microsporidia or Cryptosporidium used in this study can detect both Enterocytozoon bieneusi and Encephalitozoon intestinalis, or both Cryptosporidium hominis and Cryptosporidium parvum, respectively. Restriction enzyme digestion of PCR products with BsaBI or BsiEI makes it possible to distinguish the 2 species of microsporidia or Cryptosporidium, respectively. This simple, rapid, and cost-effective multiplex PCR method will be useful for detecting outbreaks or sporadic cases of waterborne protozoa infections.
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